535142

Protease Inhibitor Cocktail I

Animal-Free, lyophilized, for the inhibition of serine, esterases, cysteine, trypsin-like and metalloproteases. This small molecule/inhibitor is primarily used for Protease Inhibitors applications.

• Sinônimo(s): Animal-Free Protease Inhibitor Mix, Protease inhibitor
• Código UNSPSC: 12352200
• NACRES: NA.77

Propriedades

• product name: Protease Inhibitor Cocktail Set I, Animal-Free, The Protease Inhibitor Cocktail Set I, Animal-Free controls the activity of Protease. This small molecule/inhibitor is primarily used for Protease Inhibitors applications.
• forma: lyophilized
• fabricante/nome comercial: Calbiochem^®
• condição de armazenamento: OK to freeze; desiccated (hygroscopic)
• Condições de expedição: wet ice
• temperatura de armazenamento: −20°C

Descrição

Descrição geral

A cocktail of five protease inhibitors for the inhibition of a broad range of proteases and esterases. Each vial, when reconstituted with 1 ml H₂O, yields a 100X stock solution. When diluted to 1X the cocktail contains 500 µM AEBSF, HCl (Cat. No. 101500), 150 nM Aprotinin (Cat. No. 616371), 1 µM E-64 (Cat. No. 324890), 0.5 mM EDTA, Disodium Salt and 1 µM Leupeptin Hemisulfate (Cat. No. 108975). Available as 1 ml or as a set of 10 x 1 ml.

Aplicação

Protease Inhibitor Cocktail Set I, Animal-Free has been used:

• as a supplement in cell lysis buffer to extract proteins from non-small-cell lung carcinoma (NSCLC) cell lines
• in NP-40 buffer to lyse the cells for sodium dodecyl sulfate-12% polyacrylamide gel electrophoresis (SDS-PAGE) and western blotting
• as a component in lysis buffer to lyse transfected Huh7 cells and to wash the pellets to detect hepatitis B virus (HBV) C protein phosphorylation in the cell

Ações bioquímicas/fisiológicas

Protease inhibitor cocktails and specific protease inhibitors are valuable resources for scientists involved in proteomic studies, such as protein expression, characterization, biomarker discovery, post-translational modification mapping, protein profiling, and quantitative protein measurement. By adding a protease inhibitor cocktail or specific inhibitor, the integrity of proteins is preserved for subsequent analysis and further sample characterization.

Advertência

Toxicity: Standard Handling (A)

Reconstituição

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.

Reconstitute each vial in 1 ml H₂O to yield a 100X stock solution.

Informações legais

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Pictogramas: GHS08,GHS05,GHS07
• Palavra indicadora: Danger
• Frases de perigo: H314,H332,H373
• Declarações de precaução: P260 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 - P314
• Classificações de perigo: Acute Tox. 4 Inhalation - Eye Dam. 1 - Skin Corr. 1A - STOT RE 2 Inhalation
• Órgãos-alvo: Respiratory Tract
• Código de classe de armazenamento: 8A - Combustible corrosive hazardous materials
• Classe de risco de água (WGK): WGK 3