393206
Anti-HNE Fluorophore Rabbit pAb
liquid, Calbiochem®
Sinônimo(s):
Anti-N α-Acetyllysine-4-hydroxy-2-nonenal Fluorophore, Anti-Nα-Acetyllysine-4-hydroxy-2-nonenal Fluorophore
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About This Item
Código UNSPSC:
12352203
NACRES:
NA.43
Produtos recomendados
fonte biológica
rabbit
Nível de qualidade
forma do anticorpo
diluted serum
tipo de produto de anticorpo
primary antibodies
clone
polyclonal
forma
liquid
reatividade da espécie (prevista por homologia)
all
fabricante/nome comercial
Calbiochem®
condição de armazenamento
OK to freeze
avoid repeated freeze/thaw cycles
Isotipo
IgG
Condições de expedição
wet ice
temperatura de armazenamento
−70°C
modificação pós-traducional do alvo
unmodified
Descrição geral
Anti-HNE Fluorophore, rabbit polyclonal, recognizes the HNE-derived chromphohore of the 2:1 lysine-HNE-fluorophore. It is validated for use in ELISA, Western blotting, and immunohistochemistry.
Rabbit polyclonal antibody supplied as diluted serum. Recognizes the HNE-derived chromophore of the 2:1 lysine-HNE fluorophore.
Recognizes the HNE-derived chromphohore of the 2:1 lysine-HNE-fluorophore.
Imunogênio
a 2:1 Nα-acetyllysine-HNE fluorophore, conjugated to KLH
Aplicação
ELISA (1:500-1:1000)
Immunoblotting (1:1000-1:5000)
Immunohistochemistry (1:100-1:500)
Immunoblotting (1:1000-1:5000)
Immunohistochemistry (1:100-1:500)
Advertência
Toxicity: Standard Handling (A)
forma física
Serum diluted 1:1 with glycerol.
Reconstituição
Following initial thaw, aliquot and freeze (-70°C).
Outras notas
Due to the small epitope size, overnight incubation with the antibody greatly increases sensitivity by immunoblotting. Determined by competitive ELISA and immunoblotting to recognize the chromophore structure of fluorophore derived from the reaction of 4-HNE with primary amines. Does not recognize 1:1 cysteine-, 1:1 histidine-, or 1:1 lysine-HNE Michael Adducts. Variables associated with assay conditions will dictate the proper working dilution.
Tsai, L., et al. 1998. Proc. Natl. Acad. Sci. USA95, 7975.
Informações legais
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Código de classe de armazenamento
10 - Combustible liquids
Classe de risco de água (WGK)
WGK 1
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