16-454

Anti-Phosphotyrosine Antibody, clone PY20, HRP Conjugate

clone PY20, Upstate^®, from mouse

• Sinônimo(s): PY20, anti-phosphotyrosine antibody
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: mouse
• Nível de qualidade: 100
• conjugado: peroxidase conjugate
• forma do anticorpo: purified immunoglobulin
• tipo de produto de anticorpo: primary antibodies
• clone: PY20, monoclonal
• reatividade da espécie (prevista por homologia): all
• fabricante/nome comercial: Upstate^®
• técnica(s): ELISA: suitable; western blot: suitable
• Isotipo: IgG2b
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... PID1(55022)

Descrição

Descrição geral

Protein tyrosine phosphorylation was first described in 1979. Large numbers of tyrosine kinases have been described since, and many of the signaling pathways initiated by tyrosine phosphorylation have been delineated. Availability of a top-quality anti-phosphotyrosine antibody has allowed the study of tyrosine kinase activity by simple blotting procedures, and without routine need of large quantities of radioactivity. The 4G10 monoclonal anti-phosphotyrosine antibody (Cat. # 05-321) has been widely recognized as a highly specific and sensitive. This antibody is suitable for all applications involving protein tyrosine phosphorylation. The slightly less sensitive, but still highly regarded clone PY20 can be used as an alternative to the 4G10 product line.

Especificidade

Recognizes proteins containing phosphotyrosine residues, regardless of origin.

Imunogênio

Epitope: phosphotyrosine

Phosphotyrosine conjugated to carrier protein

Aplicação

Anti-Phosphotyrosine Antibody, clone PY20, HRP Conjugate is a Mouse Monoclonal Antibody for the detection of Phosphotyrosine & has been validated in WB, ELISA.

Research Category
Signaling

Research Sub Category
General Post-translation Modification

Qualidade

Routinely evaluated by western blot on RIPA lysates from insulin-treated human HEK293 cells.

Descrição-alvo

Varies

forma física

100 μL of protein A purified mouse IgG2b in PBS containing 0.05% Kathon^® and HRP stabilizing compounds. Sufficient for a minimum of 10 immunoblots, if used at the optimum concentration of 1:2000- 1:4000 in 10 mL blocking buffer. Liquid.

Protein A purified

Armazenamento e estabilidade

6 months at 2-8°C from date of shipment.
NOTE: DO NOT FREEZE. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Informações legais

Kathon is a registered trademark of Rohm and Haas Co.

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Pictogramas: GHS07,GHS09
• Palavra indicadora: Warning
• Frases de perigo: H315,H317,H319,H411
• Declarações de precaução: P261 - P264 - P273 - P280 - P302 + P352 - P305 + P351 + P338
• Classificações de perigo: Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable