07-741

Anti-AS160 (Rab-GAP) Antibody

serum, Upstate^®

• Sinônimo(s): Acrg embryonic lethality minimal region ortholog, Akt substrate of 160 kDa, TBC (Tre-2, BUB2, CDC16) domain-containing protein, TBC1 domain family, member
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: rabbit
• Nível de qualidade: 100
• forma do anticorpo: serum
• tipo de produto de anticorpo: primary antibodies
• clone: polyclonal
• reatividade de espécies: canine, human, mouse, rat
• fabricante/nome comercial: Upstate^®
• técnica(s): western blot: suitable
• Isotipo: IgG
• nº de adesão NCBI: NP_055647
• nº de adesão UniProt: O60343
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... TBC1D4(9882) ; mouse ... Tbc1D4(210789)

Descrição

Descrição geral

AS160 (Akt Substrate of 160 kDa, Akt1S1), Rab GAP (GTPase activating protein), is phosphorylated on multiple sites by Akt in response to insulin in adipocytes and contraction in muscles. AS160 is the long sought after link between the upstream insulin signaling through insulin/PI3K/Akt and GLUT4 vessicle translocation to the cell membrane. It has 6 Akt phosphorylation consensus sequences that are believed to become phosphorylated upon insulin stimulation. This phosphorylation is required for translocation of GLUT4-containing vesicles to the cell membrane1. A mutation of two or more of these phosphorylation sites results in a dramatic decrease in AS160 activity and insulin-stimulated GLUT4 redistribution at the cell membrane2. AS160 has also shown to be important for GLUT4 expcytosis2.

Especificidade

Predicted to cross react with rat and dog based on sequence homology.

Recognizes AS160 (Rab-GAP), Mr 160 kDa.

Imunogênio

KLH-conjugated, synthetic peptide corresponding to amino acids 1178-1189 (C-PTNDKAKAGNKP) of rat Akt substrate of 160kDa (AS160) (Rab-GAP) with a N-terminal cysteine for conjugation purposes.

Aplicação

Detect AS160 (Rab-GAP) using this Anti-AS160 (Rab-GAP) Antibody validated for use in WB.

Research Category
Signaling

Research Sub Category
PI3K, Akt, & mTOR Signaling

Qualidade

Routinely evaluated by Western Blot in RIPA lysates from 3T3/L1 cells differentiated into adipocytes.

Western Blot Analysis:
A 1:1000-1:5000 dilution of this lot detected AS160 (Rab-GAP) in RIPA lysates from 3T3/L1 cells differentiated into adipocytes by MDI treatment. This lot also detects AS160 (Rab-GAP) in HEK293 lysates.

Descrição-alvo

Approx. 160 kDa

forma física

Rabbit polyclonal antiserum IgG in buffer containing 0.05% sodium azide and 30% glycerol.

Unpurified

Armazenamento e estabilidade

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Nota de análise

Control
MDI-treated 3T3/L1 cell lysates.

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informações legais

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable