ABS54

Anti-AS160 Antibody

from rabbit, purified by affinity chromatography

• Sinônimo(s): Acrg embryonic lethality minimal region ortholog, Akt substrate of 160 kDa, TBC (Tre-2, BUB2, CDC16) domain-containing protein, TBC1 domain family, member
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: rabbit
• Nível de qualidade: 100
• forma do anticorpo: affinity isolated antibody
• tipo de produto de anticorpo: primary antibodies
• clone: polyclonal
• purificado por: affinity chromatography
• reatividade de espécies: mouse, human
• reatividade da espécie (prevista por homologia): rat (based on 100% sequence homology), canine (based on 100% sequence homology)
• técnica(s): immunoprecipitation (IP): suitable; western blot: suitable
• nº de adesão NCBI: NP_001074747
• nº de adesão UniProt: Q8BYJ6
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... TBC1D4(9882) ; mouse ... Tbc1D4(210789)

Descrição

Descrição geral

AS160 (Akt Substrate of 160 kDa, Akt1S1), Rab GAP (GTPase activating protein), is phosphorylated on multiple sites by Akt in response to insulin in adipocytes and contraction in muscles. AS160 is the long sought after link between the upstream insulin signaling through insulin/PI3K/Akt and GLUT4 vessicle translocation to the cell membrane. It has 6 Akt phosphorylation consensus sequences that are believed to become phosphorylated upon insulin stimulation. This phosphorylation is required for translocation of GLUT4-containing vesicles to the cell membrane1. A mutation of two or more of these phosphorylation sites results in a dramatic decrease in AS160 activity and insulin-stimulated GLUT4 redistribution at the cell membrane2. AS160 has also shown to be important for GLUT4 expcytosis2.

Especificidade

Chimpanzee, equine, and ox (90% sequence homology).

This antibody recognizes AS160 at the C-terminus.

Imunogênio

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to the C-terminus of mouse AS160.

Aplicação

Anti-AS160 Antibody detects level of AS160 & has been published & validated for use in WB & IP.

Research Category
Signaling

Research Sub Category
PI3K, Akt, & mTOR Signaling

Western Blot (SNAP ID) Analysis: 1 µg/mL from a previous lot detected AS160 on 10 µg of HEPG2 cell lysate.

Immunoprecipitation Analysis: 10 µg from a previous lot immunoprecipitated AS160 from HEPG2 lysate.

Qualidade

Evaluated by Western Blot in HEPG2 cell lysate.

Western Blot Analysis: 0.05 µg/mL of this antibody detected AS160 on 10 µg of HEPG2 cell lysate.

Descrição-alvo

~ 160 kDa

forma física

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Nota de análise

Control
HEPG2 cell lysate

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable