L021000

DCA Deblock

with Dichloromethane

• Código UNSPSC: 41116004
• NACRES: NA.21

Propriedades

• Nível de qualidade: 200
• tipo: for DNA synthesis; for DNA synthesis
• linha de produto: Proligo Reagents
• Formulário: liquid
• condição de armazenamento: dry at room temperature
• concentração: 42.2-51.6 g/L (DCA content, titration)
• Impurezas: ≤200 ppm water content (Karl Fischer)
• cor: clear

Descrição

Descrição geral

Deblock solution contains dichloroacetic acid (DCA) in dichloromethane that assists in the removal of the dimethoxytrityl (DMT) protecting group from the 5′hydroxyl moiety of nucleotides already incorporated into the growing nucleic acid, before the addition of the next phosphoramidite. Removal of the DMT allows the unprotected 5′hydroxyl moiety to react with a new phosphoramidite in a subsequent extension reaction.

Nota de preparo

Dicloromethane / Dichloroacetic acid 97/3 (v/v)

Outras notas

Deblock solution containing dichloroacetic acid (DCA) in dichloromethane removes the dimethoxytrityl (DMT) protecting group from the 5′hydroxyl moiety of nucleotides already incorporated into the growing nucleic acid, prior to the addition of the next phosphoramidite. Removal of the DMT allows the unprotected 5′hydroxyl moiety to react with a new phosphoramidite in a subsequent extension reaction.

Informações de segurança

• Pictogramas: GHS08,GHS05,GHS07
• Palavra indicadora: Danger
• Frases de perigo: H315,H318,H336,H351,H360FD,H362
• Declarações de precaução: P202 - P263 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313
• Classificações de perigo: Carc. 2 - Eye Dam. 1 - Lact. - Repr. 1B - Skin Irrit. 2 - STOT SE 3
• Órgãos-alvo: Central nervous system
• Código de classe de armazenamento: 6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable