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1.20079

Millipore

Eshmuno® Q Resin

Eshmuno® Q

Synonym(s):

Eshmuno® Q

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About This Item

UNSPSC Code:
47101511
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Quality Level

ligand

(Trimethylammoniumethyl)

sterility

sterile (Caustic Stable)

product line

Eshmuno®

form

resin

specific analyte(s)

MAbs

parameter

1000 cm/hr flow rate
1000 cm/hr max. flow rate
8 bar max. pressure

matrix active group

polymer, polyvinyl ether

mean particle size

85 μm

capacity

150 mg binding capacity (BSA/mg of resin)
~150 mg, BSA binding capacity

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This Item
MAB3120CC130ESG2206
biological source

Escherichia coli

biological source

mouse

biological source

Escherichia coli, human

biological source

-

technique(s)

cell culture | stem cell: suitable

technique(s)

ELISA: suitable, immunocytochemistry: suitable, immunofluorescence: suitable, immunohistochemistry: suitable, western blot: suitable

technique(s)

cell attachment: suitable, cell culture | mammalian: suitable, cell culture | stem cell: suitable

technique(s)

cell culture | stem cell: suitable

assay

>70% (SDS-PAGE)

assay

-

assay

≥92% (SDS-PAGE)

assay

>85% (active component rat LIF (rtLIF), SDS-PAGE)

recombinant

expressed in E. coli

recombinant

-

recombinant

-

recombinant

-

form

liquid

form

-

form

liquid

form

liquid

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

General description

Eshmuno® Q resin is a strong anion exchange resin, coupling our renowned tentacle structure with a hydrophilic polyvinyl ether base matrix. Consequently, Eshmuno® Q resin offers outstanding results in typical anion exchange applications, such as removing biomolecules′ impurities in flow-through mode.

Features and Benefits

Eshmuno® Q chromatography resin enables:

  • Purification of acidic and neutral proteins and peptides from different sources, including plasma

Superior productivity for downstream processing of biomolecules
  • High flow rate versus pressure-flow behavior
  • Excellent removal of impurities, such as HCP, leached Protein A, and viruses
  • Robust and safe packing procedures
  • Strong chemical stability

Packaging

  • 1.20079.0100: Eshmuno® Q Resin 100ml
  • 1.20079.0010: Eshmuno® Q Resin 10ml
  • 1.20079.0500: Eshmuno® Q Resin 500ml
  • 1.20079.5000: Eshmuno® Q Resin 5L

Plastic bottle

Analysis Note

Appearance: Milky cream coloured turbid suspension, free from coarse mechanical impurities.
Microscopic evaluation: Spherical particles, no agglomerates, no fines.
Extractable matter (water): ≤ 0.08 %
Ionic capacity: 90 - 190 µeq/ml
Performance test (conalbumin): 9 - 19 mS/cm
Performance test (human serum albumin): 20 - 30 mS/cm
Pressure drop(column: ID=1.6 cm, L=10 cm at 5 ml/min): ≤ 1.0 bar
Protein binding capacity(bovine serum albumin): 120 - 190 mg/ml
Particle size (50-120 µm): ≥ 80 %
Particle size (d50): 75 - 95 µm
Cerium: ≤ 30 µg/g
Endotoxins: ≤ 1.00 EU/ml
TAMC (total aerobic microbial count): ≤ 100 CFU/ml
TYMC (total yeast and mould count): ≤ 100 CFU/ml

Legal Information

Eshmuno is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Flame

signalword

Warning

hcodes

Hazard Classifications

Flam. Liq. 3

Storage Class

3 - Flammable liquids

wgk_germany

WGK 1

flash_point_f

95.0 °F

flash_point_c

35 °C


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Mian Zhang et al.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 34(4), 726-738 (2018-11-30)
Traumatic joint injuries produce osteoarthritic cartilage manifesting accelerated chondrocyte terminal differentiation and matrix degradation via unknown cellular and molecular mechanisms. Here we report the ability of biomechanical stress to increase expression of the calcium-sensing receptor (CaSR), a pivotal driver of
Stem cell engineering using transducible Cre recombinase.
Nolden, Lars, et al.
Methods in Molecular Medicine, 140, 17-32 (2007)
Site-specific recombination in human embryonic stem cells induced by cell-permeant Cre recombinase.
Nolden, Lars, et al.
Nature Methods, 3, 461-467 (2006)
Stage-specific conditional mutagenesis in mouse embryonic stem cell-derived neural cells and postmitotic neurons by direct delivery of biologically active Cre recombinase.
Haupt, Simone, et al.
Stem Cells, 25, 181-188 (2007)
Raktima Raychowdhury et al.
ImmunoHorizons, 5(10), 818-829 (2021-10-21)
In this study, we report that the TLR4 ligand, LPS, and TLR3 ligand polyinosinic:polycytidylic acid failed to activate IRF3 or STAT1 in bone marrow-derived macrophages (BMMs) isolated from two independently generated lines of Rosa26-integrated Cas9-expressing C57BL/6J (B6) mice. RNA-sequencing analysis

Protocols

Summary application report for analysis of moisture in Polyurethane glues

Summary application report for analysis of moisture in Sugar

Foodstuffs encompass the most diverse products. Depending on whether carbohydrate-rich, fatty or proteinrich substances are under investigation, different working techniques are favourable. Complex matrices that dissolve slowly, or where the water can only be slowly extracted, necessitate the use of solubilisers.

Summary application report for analysis of moisture in Sugar

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