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MAB3120

Sigma-Aldrich

Anti-Cre Recombinase Antibody, clone 2D8

ascites fluid, clone 2D8, Chemicon®

eCl@ss:
32160702
NACRES:
NA.41

Quality Level

biological source

mouse

antibody form

ascites fluid

antibody product type

primary antibodies

clone

2D8, monoclonal

species reactivity (predicted by homology)

all

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgG1κ

UniProt accession no.

shipped in

dry ice

General description

Cre-recombinase is a 38 kDa DNA recombinase derived from the P1 bacteriophage. It is highly specific for a 34 bp DNA sequence (loxP) found in P1 DNA. It catalyzes site-specific recombination between two 34-base-pair LOXP sites. Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state. It is homotetramer when bound to DNA and belongs to the "phage" integrase family. This antibody can be used to determine the success of gene manipulations in transgenic animals.

Specificity

Cre recombinase. The epitope is located between aa 77 and 343.

Immunogen

Cre recombinase (aa 77 to 343) fusion protein

Application

Research Sub Category
Cell Cycle, DNA Replication & Repair
Western Blot (natural and recombinant protein):
A 1:500-1:5,000 dilution was used in a previous lot. Natural Cre recombinase from Bacteriophage P1 migrates at 38.5 kDa by SDS-PAGE.

Immunofluorescence:
A previous lot of this antibody was used on IF.

Immunohistochemistry:
A 1:500-1:5,000 dilution of a previous lot was used in IH.

ELISA:
A 1:500-1:5,000 dilution of a previous lot was used in ELISA.

Optimal working dilutions must be determined by the end user.
Anti-Cre Recombinase Antibody, clone 2D8 is a well characterized monoclonal antibody. Proven to reliably detect Cre Recombinase, this mAb is validated for use in ELISA, IC, IF, IH & WB and is backed by multiple publications.
Research Category
Epigenetics & Nuclear Function

Target description

38 kDa

Physical form

Ascites fluid. Contains no preservative
Unpurified

Storage and Stability

Stable for 1 year at -20ºC from date of receipt.

Analysis Note

Control
HeLa Nuclear Extract

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Quality

Enter Lot Number to search for Certificate of Quality (COQ).

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