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T9424

Sigma-Aldrich

TRI Reagent®

For processing tissues, cells cultured in monolayer or cell pellets

Synonym(s):
DNA/RNA/protein extraction reagent, total RNA purification solution, cell pellet RNA purification, single step RNA isolation reagent, TRI Reagent cell pellet solution, DNA/RNA/protein isolation reagent, cell pellet RNA extraction, total RNA extraction solution, cell pellet RNA isolation, DNA/RNA/protein purification reagent, total RNA isolation solution, single step RNA purification reagent, TRI Reagent RNA Isolation Reagent, single step RNA extraction reagent
MDL number:
NACRES:
NA.52

Quality Level

usage

 mL sufficient for 107 cells
 mL sufficient for 100 mg tissue (or)

storage temp.

2-8°C

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General description

TRI reagent® is a quick and convenient ready-to-use reagent useful for efficient total RNA extraction or for the simultaneous isolation of RNA, DNA and protein. TRI reagent® is a mixture of guanidine thiocyanate and phenol in a monophasic solution.
Homogenization or lysis of the tissue sample in TRI reagent® dissolves RNA, DNA and protein. The addition of chloroform or 1-bromo-3-chloropropane followed by centrifugation results in the separation of the mixture into three phases: an aqueous phase containing the RNA, the interphase containing DNA, and an organic phase containing proteins. Each of the components can then be isolated after separating the phases.

Application

TRI Reagent has been used:
  • in the isolation of total RNA from various tissue samples and cells
  • for the lysis of monocytes
  • to isolate cytoplasmic RNA from SARS-CoV infected Vero cells
  • to isolate total RNA from MDMs (monocyte-derived macrophages) infected with influenza A (H5N1) virus
  • to prepare a viral lysate of SARS-CoV culture for the creation of a clone encoding the S glycoprotein in the development of SARS-CoV vaccine

The RNA, DNA and protein that are isolated using TRI reagent® can further be used for downstream applications, such as cloning, PCR, RT-PCR, Northern blots, mRNA isolation, in vitro translation, RNase protection assay, restriction enzyme digestion, Southern blots, SDS-PAGE and western blots.
TRI Reagent is an improved version of the single-step total RNA isolation reagent developed by Chomczynski. The RNA isolation method based on this reagent is widely used and proven for RNA applications. It is ideal for quick, economical, and efficient isolation of total RNA or the simultaneous isolation of RNA, DNA, and proteins from samples of human, animal, plant, yeast, bacterial, and viral origin.

Packaging

25, 100, 200 mL in glass bottle

Features and Benefits

Easy-to-use: A single reagent that can be used for RNA, DNA and protein isolation that is free of any contamination.
Multi-purpose: TRI Reagent® performs well with large or small amounts of tissue or cells and works with many samples including human, plant, yeast, bacterial and viral samples.
Efficient: TRI reagent® gives better yields than traditional guanidine thiocyanate/cesium chloride methods. The whole process of RNA extraction starting with fresh tissue or cells can be completed in less than one hour.
  • Easily scalable RNA isolation
  • Works with many sources: human, plant, yeast, bacterial, or viral
  • Better yields than traditional guanidine thiocyanate/cesium chloride methods

Legal Information

TRI Reagent is a registered trademark of Molecular Research Center, Inc.

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2

Supplementary Hazards

Storage Class Code

6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 2

Flash Point(F)

174.2 °F - closed cup

Flash Point(C)

79 °C - closed cup

Certificate of Analysis

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Certificate of Origin

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Articles

Genomic DNA Sample Purification and Quality Assessment

The availability of simple methods for purification of DNA and RNA has greatly facilitated the analysis and characterization of the genome and gene expression. There is a demand to isolate DNA and RNA rapidly and conveniently from a variety of cellular sources, including cells and tissues from mammalian, plant and bacterial cultures.

Protocols

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TRI Reagent® Protocol

TRI Reagent is a single-step RNA isolation reagent that allows simultaneous isolation of DNA, RNA and protein. This protocol outlines sample prep as well as extraction of the various samples and troubleshooting.

Protocol for Anti Ago-RNA Immunoprecipitation from Mammalian Cells Using the RIP Kit

Procedure and protocol for Anti Ago-RNA Immunoprecipitation from mammalian cells using the RIP kit

Northern and Southern Blot Protocols & Introduction

An introduction to both Northern and Southern blotting, popular methods for the transfer of macromolecules to membranous support. This article also offers a Southern blot protocol and a northern blot protocol.

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