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T3934

Sigma-Aldrich

TRI Reagent®

LS, For processing fluid samples such as cell suspensions, CSF, and amniotic fluid.

Synonym(s):
DNA/RNA/protein extraction reagent, total RNA purification solution, cell CSF amniotic fluid RNA extraction reagent, single step RNA isolation reagent, DNA/RNA/protein isolation reagent, cell CSF amniotic fluid RNA purification reagent, total RNA extraction solution, DNA/RNA/protein purification reagent, cell CSF amniotic fluid RNA isolation reagent, total RNA isolation solution, single step RNA purification reagent, TRI Reagent RNA Isolation Reagent, single step RNA extraction reagent
MDL number:

usage

0.75 mL sufficient for 0.25 mL fluid samples

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General description

TRI Reagent LS is a quick and convenient reagent for use in the simultaneous isolation of RNA, DNA, and protein from liquid samples of human, animal, plant, yeast, bacterial, and viral origin.

A convenient single-step liquid phase separation results in the simultaneous isolation of RNA, DNA, and protein. This procedure is an adaptation of the singlestep method reported by Chomczynski and Sacchi for total RNA isolation, and permits fast and efficient processing of liquid samples. TRI Reagent LS performs well with large or small sample volumes, and many samples can be simultaneously extracted.

TRI Reagent LS is a mixture of guanidine thiocyanate and phenol in a monophase solution. When a biological sample is homogenized or lysed with it, and chloroform or 1-bromo-3-chloropropane is added, the mixture separates into 3 phases: an aqueous phase containing RNA, the interphase containing DNA, and an organic phase containing proteins. Each component can then be isolated after separating the phases. 0.75 ml of TRI Reagent LS processes 0.25 ml of a liquid sample such as amniotic fluid.

This is one of the most effective methods for isolating total RNA from fresh samples in only one hour. The procedure is very effective for isolating RNA molecules of all types from 0.1-15 kb in length. The resulting RNA is intact with little or no contaminating DNA and protein.

Application

The reagent has been used for isolating total RNA. The resulting RNA can be used for Northern blots, mRNA isolation, in vitro translation, RNase protection assay, cloning, and PCR.
TRI Reagent® has been used in RNA extraction.
TRI Reagent is an improved version of the single-step total RNA isolation reagent developed by Chomczynski. The RNA isolation method based on this reagent is widely used and proven for RNA applications. It is ideal for quick, economical, and efficient isolation of total RNA or the simultaneous isolation of RNA, DNA, and proteins from samples of human, animal, plant, yeast, bacterial, and viral origin.

Packaging

100, 200 mL in glass bottle

Features and Benefits

  • Easily scalable RNA isolation
  • Works with many sources: human, plant, yeast, bacterial, or viral
  • Better yields than traditional guanidine thiocyanate/cesium chloride methods

Legal Information

TRI Reagent is a registered trademark of Molecular Research Center, Inc.

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2

Supplementary Hazards

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Flash Point(F)

174.2 °F - closed cup

Flash Point(C)

79 °C - closed cup

Certificate of Analysis

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Certificate of Origin

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Articles

Genomic DNA Sample Purification and Quality Assessment

The availability of simple methods for purification of DNA and RNA has greatly facilitated the analysis and characterization of the genome and gene expression. There is a demand to isolate DNA and RNA rapidly and conveniently from a variety of cellular sources, including cells and tissues from mammalian, plant and bacterial cultures.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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