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I9516

Sigma-Aldrich

2-Propanol

BioReagent, for molecular biology, ≥99.5%

Synonym(s):
sec-Propyl alcohol, IPA, Isopropanol, Isopropyl alcohol
Linear Formula:
(CH3)2CHOH
CAS Number:
Molecular Weight:
60.10
Beilstein:
635639
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.52

grade

for molecular biology

Quality Level

vapor density

2.1 (vs air)

vapor pressure

33 mmHg ( 20 °C)
44 mmHg ( 25 °C)

product line

BioReagent

assay

≥99.5%

form

liquid

autoignition temp.

750 °F

expl. lim.

2.0-12.7 %, 93 °C

technique(s)

DNA extraction: suitable
RNA extraction: suitable

impurities

≤0.2% water
water

color

colorless

refractive index

n20/D 1.377 (lit.)

bp

82 °C (lit.)

mp

−89.5 °C (lit.)

solubility

water: soluble (completely)

density

0.785 g/mL at 25 °C (lit.)

suitability

suitable for precipitation of nucleic acids

storage temp.

room temp

SMILES string

CC(C)O

InChI

1S/C3H8O/c1-3(2)4/h3-4H,1-2H3

InChI key

KFZMGEQAYNKOFK-UHFFFAOYSA-N

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General description

Isopropanol is a clear, colorless, polar organic solvent commonly used in chemistry and molecular biology laboratories. It will dissolve a wide range of chemicals, and evaporates quickly. This product (2-propanol) is designated as molecular biology grade and is suitable for the precipitation of nucleic acids.

Protocols for the use of isopropanol:

  • DNA precipitation from mammalian tissue, ethidium bromide extraction from DNA samples, and RNA isolation from cells and tissues.
[21]
  • Protein crystallization.
  • [01][23][95]
  • Isolate retinoids from biological matrices for HPLC and HPLC-MS analysis.
  • [24][20]

    Application

    2-Propanol has been used:
    • in DNA and RNA extraction
    [17][93][73][09]
  • for determination of hydroxyproline levels(The Merck Index, 12th ed)
  • for checking cell viability by MTT assay
  • [94]
  • in microarray hybridization
  • [95]Suitable for use in DNA precipitation using standard protocols. Also suitable as a solvent for making solutions for molecular biology applications.

    Packaging

    25, 500 mL in glass bottle

    Pictograms

    FlameExclamation mark

    Signal Word

    Danger

    Hazard Statements

    Hazard Classifications

    Eye Irrit. 2 - Flam. Liq. 2 - STOT SE 3

    Target Organs

    Respiratory system

    Storage Class Code

    3 - Flammable liquids

    WGK

    WGK 1

    Flash Point(F)

    53.6 °F - closed cup

    Flash Point(C)

    12.0 °C - closed cup

    Personal Protective Equipment

    dust mask type N95 (US), Eyeshields, Gloves

    Certificate of Analysis

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    Certificate of Origin

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