Skip to Content
MilliporeSigma
All Photos(2)

Key Documents

View All Documentation

B7906

Sigma-Aldrich

1-Butanol

for molecular biology, ≥99%

Synonym(s):

n-Butanol, Butyl alcohol

Sign Into View Organizational & Contract Pricing
All Photos(2)

About This Item

Linear Formula:
CH3(CH2)3OH
CAS Number:
71-36-3
Molecular Weight:
74.12
Beilstein/REAXYS Number:
969148
EC Number:
200-751-6
MDL number:
MFCD00002964
UNSPSC Code:
12352200
PubChem Substance ID:
24892030
NACRES:
NA.52

grade

for molecular biology

Quality Level

100

vapor density

2.55 (vs air)

vapor pressure

5 (4 mmHg) at 20 °C hPa

assay

≥99%

form

liquid

autoignition temp.

649 °F

expl. lim.

11.2 %

color

colorless

refractive index

n20/D 1.399 (lit.)

pH

7 (20 °C, 70 g/L)

bp

116-118 °C (lit.)

mp

−90 °C (lit.)

density

0.81 g/mL at 25 °C (lit.)

storage temp.

room temp

SMILES string

CCCCO

InChI

1S/C4H10O/c1-2-3-4-5/h5H,2-4H2,1H3

InChI key

LRHPLDYGYMQRHN-UHFFFAOYSA-N

Looking for similar products? Visit Product Comparison Guide

General description

1-Butanol is a clear, colorless alcohol that can be used for the removal of ethidium bromide from DNA purified by CsCl gradient ultracentrifugation. It is also suitable for use in the concentration of dilute nucleic acid solutions by repeated extractions, thus improving recovery by ethanol precipitation.

Application

1-Butanol has been used:
  • in the derivatization process for measuring methylated arginine levels in blood samples
  • in liquid-chromatography mass spectrometry (LC-MS) analysis of lysine modifications by deacetylase CobB
  • in the extraction of plant material from Vigna unguiculata
  • to extract and purify amplified human DNA samples before further amplification by PCR followed by sequencing

1-Butanol is suitable for use in nucleic acid purification and concentration protocols.

Related product

Product No.
Description
Pricing

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

3 - Flammable liquids

wgk_germany

WGK 1

flash_point_f

95.0 °F - Pensky-Martens closed cup

flash_point_c

35 °C - Pensky-Martens closed cup

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Pietro Benettoni et al.
Biointerphases, 15(2), 021005-021005 (2020-03-28)
Nowadays, high-resolution imaging techniques are extensively applied in a complementary way to gain insights into complex phenomena. For a truly complementary analytical approach, a common sample carrier is required that is suitable for the different preparation methods necessary for each
Hon Sing Leong et al.
Nature protocols, 5(8), 1406-1417 (2010-07-31)
Viral nanoparticles are a novel class of biomolecular agents that take advantage of the natural circulatory and targeting properties of viruses to allow the development of therapeutics, vaccines and imaging tools. We have developed a multivalent nanoparticle platform based on
Elena Castellanos-Rizaldos et al.
PloS one, 7(12), e51362-e51362 (2012-12-14)
Multiplex detection of low-level mutant alleles in the presence of wild-type DNA would be useful for several fields of medicine including cancer, pre-natal diagnosis and infectious diseases. COLD-PCR is a recently developed method that enriches low-level mutations during PCR cycling
Mehmet Nuri Atalar et al.
Life sciences, 231, 116577-116577 (2019-06-19)
Galectin-3 is associated with the process of inflammation and fibrosis. The aim of this study was both to evaluate of galectin-3, methylated arginines and hs-CRP in subjects with type 2 diabetes and prediabetes and to investigate a relation between serum
A L Gainer et al.
Clinica chimica acta; international journal of clinical chemistry, 123(1-2), 11-17 (1982-08-04)
Neutrophils were isolated in good yield from fresh whole blood and their alkaline phosphatase was solubilized. Inhibitor studies using L-phenylalanylglycylglycine, L-phenylalanine and L-homoarginine revealed a distinct pattern of inhibition for each of the crude or purified preparations of the human
View All Related Papers

Protocols

Ethidium Bromide Removal

Removal of Ethidium Bromide from DNA by Extraction with Organic Solvents Protocol from Cold Spring Harbor Laboratory Press.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service