B7906

1-Butanol

for molecular biology, ≥99%

• Synonym(s): n-Butanol, Butyl alcohol
• Linear Formula: CH₃(CH₂)₃OH
• CAS Number: 71-36-3
• Molecular Weight: 74.12
• Beilstein: 969148
• EC Number: 200-751-6
• MDL number: MFCD00002964
• PubChem Substance ID: 24892030
• NACRES: NA.52

Properties

• grade: for molecular biology
• Quality Level: 100
• vapor density: 2.55 (vs air)
• vapor pressure: 5 (4 mmHg) at 20 °C hPa
• Assay: ≥99%
• form: liquid
• autoignition temp.: 649 °F
• expl. lim.: 11.2 %
• color: colorless
• refractive index: n20/D 1.399 (lit.)
• pH: 7 (20 °C, 70 g/L)
• bp: 116-118 °C (lit.)
• mp: −90 °C (lit.)
• density: 0.81 g/mL at 25 °C (lit.)
• storage temp.: room temp
• SMILES string: CCCCO
• InChI: 1S/C4H10O/c1-2-3-4-5/h5H,2-4H2,1H3
• InChI key: LRHPLDYGYMQRHN-UHFFFAOYSA-N

Description

General description

1-Butanol is a clear, colorless alcohol that can be used for the removal of ethidium bromide from DNA purified by CsCl gradient ultracentrifugation. It is also suitable for use in the concentration of dilute nucleic acid solutions by repeated extractions, thus improving recovery by ethanol precipitation.

Application

1-Butanol has been used:

• in the derivatization process for measuring methylated arginine levels in blood samples
• in liquid-chromatography mass spectrometry (LC-MS) analysis of lysine modifications by deacetylase CobB
• in the extraction of plant material from Vigna unguiculata
• to extract and purify amplified human DNA samples before further amplification by PCR followed by sequencing

1-Butanol is suitable for use in nucleic acid purification and concentration protocols.

Safety Information

• Pictograms: GHS02,GHS05,GHS07
• Signal Word: Danger
• Hazard Statements: H226 - H302 - H315 - H318 - H335 - H336
• Precautionary Statements: P210 - P233 - P280 - P301 + P312 - P303 + P361 + P353 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Skin Irrit. 2
• Storage Class Code: 3 - Flammable liquids
• WGK: WGK 1
• Flash Point(F): 95.0 °F - Pensky-Martens closed cup
• Flash Point(C): 35 °C - Pensky-Martens closed cup
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves