MilliporeSigma
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I9392

Sigma-Aldrich

3-Methylbutanol

BioReagent, for molecular biology, ≥98.5%

Synonym(s):
Isopentyl alcohol, Isoamyl alcohol, 3-Methyl-1-butanol
Linear Formula:
(CH3)2CHCH2CH2OH
CAS Number:
Molecular Weight:
88.15
Beilstein:
1718835
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.52

Quality Level

grade

for molecular biology

product line

BioReagent

assay

≥98.5%

form

liquid

technique(s)

RNA extraction: suitable

pH

5.6 (20 °C, 25 g/L)

density

0.809 g/mL at 20 °C (lit.)

suitability

suitable for nucleic acid purification

storage temp.

room temp

SMILES string

CC(C)CCO

InChI

1S/C5H12O/c1-5(2)3-4-6/h5-6H,3-4H2,1-2H3

InChI key

PHTQWCKDNZKARW-UHFFFAOYSA-N

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General description

3-methylbutanol (isoamyl alcohol) is a clear, colorless alcohol commonly used as an organic solvent . It can also be used in phenol-chloroform nucleic acid extraction and purification protocols, where it inhibits RNase activity and reduces foaming. Isoamyl alcohol can also be used for the removal of ethidium bromide from DNA purified by CsCl gradient ultracentrifugation.

Application

3-Methylbutanol has been used as a component of phenol/chloroform/ isoamyl alcohol solution for DNA extraction from blood and from absorbing substrata based forensic samples and biofilms.
3-Methylbutanol (isoamyl alcohol) in combination with phenol and chloroform is used in extraction of RNA. It was used in the synthesis of oligo-RNAs using photocaged adenosine molecules.

Packaging

1 L in glass bottle
25, 500 mL in glass bottle

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Inhalation - Eye Dam. 1 - Flam. Liq. 3 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

3 - Flammable liquids

WGK

WGK 1

Flash Point(F)

110.3 °F - closed cup

Flash Point(C)

43.5 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Steven G Chaulk et al.
Nature protocols, 2(5), 1052-1058 (2007-06-05)
This protocol describes a general method for the preparation of RNAs in which the reactivity or hydrogen-bonding properties of the molecule are modified in a photoreversible fashion by use of a caging strategy. A single caged adenosine, modified at the
Frédéric Ravyts et al.
Food microbiology, 27(7), 945-954 (2010-08-07)
Differences in the production of bacterial metabolites with potential impact on fermented sausage flavour were found in meat simulation medium when comparing different strains of Staphylococcus xylosus and Staphylococcus carnosus as starter cultures. Overall, higher levels of 3-methyl-1-butanol and acetoin
Niels O Verhulst et al.
Malaria journal, 10, 28-28 (2011-02-10)
Anopheles gambiae sensu stricto is considered to be highly anthropophilic and volatiles of human origin provide essential cues during its host-seeking behaviour. A synthetic blend of three human-derived volatiles, ammonia, lactic acid and tetradecanoic acid, attracts A. gambiae. In addition
Mary J Dunlop et al.
Molecular systems biology, 7, 487-487 (2011-05-11)
Many compounds being considered as candidates for advanced biofuels are toxic to microorganisms. This introduces an undesirable trade-off when engineering metabolic pathways for biofuel production because the engineered microbes must balance production against survival. Cellular export systems, such as efflux
Kazushi Yoshida et al.
Nature communications, 3, 739-739 (2012-03-15)
The same odorant can induce attractive or repulsive responses depending on its concentration in various animals including humans. However, little is understood about the neuronal basis of this behavioural phenomenon. Here we show that Caenorhabditis elegans avoids high concentrations of

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