SRP6065

Hexokinase 1 human

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

• Synonym(s): Brain form hexokinase, HK1, Hexokinase type I, N/A
• CAS Number: 1713304-81-4
• UNSPSC Code: 12352204
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in E. coli
• assay: ≥90% (SDS-PAGE)
• form: liquid
• potency: 7-8 units/mL
• mol wt: 104.6 kDa
• packaging: pkg of 100 μg
• storage condition: avoid repeated freeze/thaw cycles
• NCBI accession no.: P19367
• UniProt accession no.: P19367
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... HXK1(3098)

Description

General description

Hexokinase exists as three isozymes, namely type I, type II and type III that differ in subcellular localization, catalytic and regulatory properties. The type I isozyme serves a catabolic function facilitating the entry of glucose into glycolytic metabolism for energy production. The type II and type III enzymes perform anabolic functions, such as generating G6P for glycogen synthesis or metabolism through the phosphogluconate pathway for lipid synthesis. Type I and type II isozymes have been found to be bound to mitochondria. The type III isozyme has been found to have perinuclear localization in several cell types.

Biochem/physiol Actions

Hexokinase catalyzes the conversion of hexoses to hexose-6-phosphate in an ATP-dependent manner. It is also called the glycolytic enzyme as it catalyzes the first step in glucose metabolism with the formation of glucose-6-phosphate (G6P). In eukaryotes, it functions as a glucose sensor.

Physical form

1 mg/mL solution in 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol.

Preparation Note

Centrifuge the vial prior to opening.

Other Notes

MGSSHHHHHH SSGLVPRGSH MIAAQLLAYY FTELKDDQVK KIDKYLYAMR LSDETLIDIM TRFRKEMKNG LSRDFNPTAT VKMLPTFVRS IPDGSEKGDF IALDLGGSSF RILRVQVNHE KNQNVHMESE VYDTPENIVH GSGSQLFDHV AECLGDFMEK RKIKDKKLPV GFTFSFPCQQ SKIDEAILIT WTKRFKASGV EGADVVKLLN KAIKKRGDYD ANIVAVVNDT VGTMMTCGYD DQHCEVGLII GTGTNACYME ELRHIDLVEG DEGRMCINTE WGAFGDDGSL EDIRTEFDRE IDRGSLNPGK QLFEKMVSGM YLGELVRLIL VKMAKEGLLF EGRITPELLT RGKFNTSDVS AIEKNKEGLH NAKEILTRLG VEPSDDDCVS VQHVCTIVSF RSANLVAATL GAILNRLRDN KGTPRLRTTV GVDGSLYKTH PQYSRRFHKT LRRLVPDSDV RFLLSESGSG KGAAMVTAVA YRLAEQHRQI EETLAHFHLT KDMLLEVKKR MRAEMELGLR KQTHNNAVVK MLPSFVRRTP DGTENGDFLA LDLGGTNFRV LLVKIRSGKK RTVEMHNKIY AIPIEIMQGT GEELFDHIVS CISDFLDYMG IKGPRMPLGF TFSFPCQQTS LDAGILITWT KGFKATDCVG HDVVTLLRDA IKRREEFDLD VVAVVNDTVG TMMTCAYEEP TCEVGLIVGT GSNACYMEEM KNVEMVEGDQ GQMCINMEWG AFGDNGCLDD IRTHYDRLVD EYSLNAGKQR YEKMISGMYL GEIVRNILID FTKKGFLFRG QISETLKTRG IFETKFLSQI ESDRLALLQV RAILQQLGLN STCDDSILVK TVCGVVSRRA AQLCGAGMAA VVDKIRENRG LDRLNVTVGV DGTLYKLHPH FSRIMHQTVK ELSPKCNVSF LLSEDGSGKG AALITAVGVR LRTEASS

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable