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Hexokinase from Saccharomyces cerevisiae

Type F-300, lyophilized powder, ≥130 units/mg protein (biuret)

ATP:D-Hexose-6-phosphotransferase, Hexokinase from yeast
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:


Type F-300

Quality Level


lyophilized powder

specific activity

≥130 units/mg protein (biuret)

mol wt

110 kDa

purified by


anion traces

sulfate (SO42-): free

UniProt accession no.

foreign activity

ATPase, myokinase, glucose-6-phosphate dehydrogenase, 6-phosphogluconic dehydrogenase and phosphoglucose isomerase ≤0.01%

storage temp.


Gene Information

bakers yeast ... HXK1(850614) , HXK2(852639)

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Hexokinase has been used:
  • for digesting glucose for measuring glucose content in the root and stem samples of Quercus velutina
  • to treat ADP (adenosine diphosphate) solution along with D-glucose to remove the contaminating ATP (adenosine triphosphate)
  • [84]
  • in 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid buffer (pH 7.5) for ADP-[13CU]glucose synthesis
  • [93]


    500, 1000 units in glass bottle
    2500, 10000 units in poly bottle

    Biochem/physiol Actions

    Catalyzes the phosphorylation of D-hexose sugars at the C6 position utilizing ATP as a phosphate source.

    The rate of phosphorylation varies with different hexoses (pH 7.5, 30 °C).
    D-fructose KM: 0.33 mM
    D-glucose KM: 0.12 mM
    D-mannose KM: 0.05 mM

    Yeast hexokinase exists as two similar isoforms, PI and PII (A and B), with isoelectric points of 5.25 and 4, respectively.

    Molecular Weight: ~ 54 kDa (monomer)
    ~110 kDa (dimer)
    Optimal pH: 7.5 to 9.0
    Extinction Coefficient: E1% = 8.85 (PI) and 9.47 (PII) at 280 nm

    Activators: Hexokinase requires Mg2+ ions (KM = 2.6 mM) for activity. Hexokinase is activated by catecholamines and related compounds.

    Inhibitors: sorbose-1-phosphate, polyphosphates, 6-deoxy-6-fluoroglucose, 2-C-hydroxy-methylglucose, xylose, lyxose, and thiol reactive compounds (Hg2+ and 4-chloromercuribenzoate)


    A mixture of isoenzymes.

    Unit Definition

    One unit will phosphorylate 1.0 μmole of D-glucose per min at pH 7.6 at 25 °C.

    Physical form

    Lyophilized powder containing approx. 15% sodium citrate


    Reconstitute with water or citrate buffer, pH 7.0. Studies have shown excellent stability during repeated freezing and thawing over a period of at least 30 days.

    Storage Class Code

    13 - Non Combustible Solids


    WGK 3

    Flash Point(F)

    Not applicable

    Flash Point(C)

    Not applicable

    Personal Protective Equipment

    dust mask type N95 (US), Eyeshields, Gloves

    Certificate of Analysis

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    Certificate of Origin

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    Technical Information


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