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H4502

Sigma-Aldrich

Hexokinase from Saccharomyces cerevisiae

Type F-300, lyophilized powder, ≥130 units/mg protein (biuret)

Synonym(s):
ATP:D-Hexose-6-phosphotransferase, Hexokinase from yeast
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
eCl@ss:
32160410
NACRES:
NA.54

type

Type F-300

Quality Level

form

lyophilized powder

specific activity

≥130 units/mg protein (biuret)

mol wt

110 kDa

purified by

crystallization

anion traces

sulfate (SO42-): free

UniProt accession no.

foreign activity

ATPase, myokinase, glucose-6-phosphate dehydrogenase, 6-phosphogluconic dehydrogenase and phosphoglucose isomerase ≤0.01%

storage temp.

−20°C

Gene Information

bakers yeast ... HXK1(850614) , HXK2(852639)

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Application

Hexokinase has been used:
  • for digesting glucose for measuring glucose content in the root and stem samples of Quercus velutina
[42]
  • to treat ADP (adenosine diphosphate) solution along with D-glucose to remove the contaminating ATP (adenosine triphosphate)
  • [84]
  • in 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid buffer (pH 7.5) for ADP-[13CU]glucose synthesis
  • [93]

    Packaging

    500, 1000 units in glass bottle
    2500, 10000 units in poly bottle

    Biochem/physiol Actions

    Catalyzes the phosphorylation of D-hexose sugars at the C6 position utilizing ATP as a phosphate source.

    The rate of phosphorylation varies with different hexoses (pH 7.5, 30 °C).
    D-fructose KM: 0.33 mM
    D-glucose KM: 0.12 mM
    D-mannose KM: 0.05 mM

    Yeast hexokinase exists as two similar isoforms, PI and PII (A and B), with isoelectric points of 5.25 and 4, respectively.

    Molecular Weight: ~ 54 kDa (monomer)
    ~110 kDa (dimer)
    Optimal pH: 7.5 to 9.0
    Extinction Coefficient: E1% = 8.85 (PI) and 9.47 (PII) at 280 nm

    Activators: Hexokinase requires Mg2+ ions (KM = 2.6 mM) for activity. Hexokinase is activated by catecholamines and related compounds.

    Inhibitors: sorbose-1-phosphate, polyphosphates, 6-deoxy-6-fluoroglucose, 2-C-hydroxy-methylglucose, xylose, lyxose, and thiol reactive compounds (Hg2+ and 4-chloromercuribenzoate)

    Quality

    A mixture of isoenzymes.

    Unit Definition

    One unit will phosphorylate 1.0 μmole of D-glucose per min at pH 7.6 at 25 °C.

    Physical form

    Lyophilized powder containing approx. 15% sodium citrate

    Reconstitution

    Reconstitute with water or citrate buffer, pH 7.0. Studies have shown excellent stability during repeated freezing and thawing over a period of at least 30 days.

    Storage Class Code

    13 - Non Combustible Solids

    WGK

    WGK 3

    Flash Point(F)

    Not applicable

    Flash Point(C)

    Not applicable

    Personal Protective Equipment

    dust mask type N95 (US), Eyeshields, Gloves

    Certificate of Analysis

    Enter Lot Number to search for Certificate of Analysis (COA).

    Certificate of Origin

    Enter Lot Number to search for Certificate of Origin (COO).

    Technical Information

    SDS

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