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RPROB

Sigma-Aldrich

Reversible Protein Detection Kit for Membranes and Polyacrylamide Gels

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

usage

sufficient for 20 applications staining (small 8 X 10 cm blots or PAGE gels)

Quality Level

200

technique(s)

protein staining: suitable

storage temp.

2-8°C

Application

R-PROB is a unique protein detection system designed for staining of proteins on nylon, nitrocellulose, and PVDF membranes or PAGE gels with the detection sensitivity similar to that of Coomassie stains. Most other stains produce high backgrounds on nylon membranes due to strong charge interactions with the membrane. The mixing of equal amounts of Solution A and B activates the stain, which produces lavender stained protein bands. The staining is easily reversible with an EDTA solution so that the blot can be reused for Western blotting or for amino acid sequence analysis (PVDF). R-PROB does not contain the toxic chemicals cacodylic acid or potassium ferrocyanide.

Other Notes

Compatible with nylon, nitrocellulose, and PVDF membranes as well as polyacrylamide gels.

Kit Components Only

Product No.
Description
  • Solution A 500 mL
  • Solution B 500 mL

pictograms

Corrosion
GHS05

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2

Storage Class

8B - Non-combustible corrosive hazardous materials

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Sensitivity and mass accuracy for proteins analyzed directly from polyacrylamide gels: Implications for proteome mapping
Rachel R.
Electrophoresis (1997)

Articles

Electrophoresis Protein Staining Reagents Selection Guide

To meet the great diversity of protein analysis needs, Sigma offers a wide selection of protein visualization (staining) reagents. EZBlue™ and ProteoSilver™, designed specifically for proteomics, also perform impressively in traditional PAGE formats.

Protocols

Introduction to Western Blot Detection of Proteins

Western blotting involves protein separation by gel electrophoresis, protein transfer to a polyvinylidene difluoride (PVDF) or nitrocellulose membrane, and detection by various methods.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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