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P7170

Sigma-Aldrich

Ponceau S solution

BioReagent, suitable for electrophoresis, 0.1 % (w/v) in 5% acetic acid

Empirical Formula (Hill Notation):
C22H12N4Na4O13S4
CAS Number:
Molecular Weight:
760.57
MDL number:
PubChem Substance ID:

Quality Level

product line

BioReagent

form

liquid

concentration

0.1 % (w/v) in 5% acetic acid

application(s)

hematology: suitable
histology: suitable

density

1.01 g/cm3 at 20 °C

suitability

suitable for electrophoresis

Featured Industry

Diagnostic Assay Manufacturing

storage temp.

room temp

SMILES string

[Na+].[Na+].[Na+].[Na+].Oc1c(\N=N\c2ccc(cc2S([O-])(=O)=O)\N=N\c3ccc(cc3)S([O-])(=O)=O)c4ccc(cc4cc1S([O-])(=O)=O)S([O-])(=O)=O

InChI

1S/C22H16N4O13S4.4Na/c27-22-20(43(37,38)39)10-12-9-16(41(31,32)33)6-7-17(12)21(22)26-25-18-8-3-14(11-19(18)42(34,35)36)24-23-13-1-4-15(5-2-13)40(28,29)30;;;;/h1-11,27H,(H,28,29,30)(H,31,32,33)(H,34,35,36)(H,37,38,39);;;;/q;4*+1/p-4/b24-23+,26-25+;;;;

InChI key

KQHKSGRIBYJYFX-WNDMICSESA-J

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General description

Ponceau S is the sodium salt of a diazo dye. Ponceau S solution is a suitable reagent for use in electrophoresis studies. The Ponceau S stain comes ready-to-use and is designed for rapid (5 min) staining of protein bands on nitrocellulose or PVDF membranes (Western blots) and also for staining protein on cellulose acetate membranes. It may be employed as stain for the purified fibrinolytic enzyme (CK) for the analysis of N-terminal amino acid sequence of the first 14 residues of CK by a protein sequencer. Ponceau S staining on nitrocellulose and PVDF is reversible by washing the stained membrane with 0.1M NaOH for 1 minute. The stained and destained membrane can then be immunologically detected (Western blotting). A product information sheet is supplied which includes the staining procedure for cellulose acetate, nitrocellulose, and PVDF.

Application

Ponceau S solution has been used for membrane staining (Western blotting).

Packaging

1 L in poly bottle

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

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Immunomodulatory effect of arsenic on cytokine and HSP gene
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Fish & Shellfish Immunology, 44 (2015)
Joshua P Nederveen et al.
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PLoS neglected tropical diseases, 12(9), e0006792-e0006792 (2018-09-18)
The protozoan parasite Trypanosoma cruzi, the causative agent of Chagas disease, causes severe morbidity and mortality in afflicted individuals. About 30% of T. cruzi-infected individuals present with cardiac, gastrointestinal tract, and/or neurological disorders. Megacolon, one of the major pathologies of...
Legume Grains Enhance Ileal Losses of Specific Endogenous Serine-Protease Proteins in Weaned Pigs1
Paulo Salgado
The Journal of Nutrition (2002)

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