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PROTSIL1

Sigma-Aldrich

ProteoSilver Silver Stain Kit

Synonym(s):
Silver Staining Kit

usage

 kit sufficient for 25 mini-gels (10 × 10 cm)

Quality Level

technique(s)

protein staining: suitable

General description

Conveniently packaged, extremely stable, and highly sensitive, ProteoSilver is an ideal product for any protein scientist. The kit contains prepared solutions of silver staining reagents along with detailed instructions to achieve optimal results. With a detection limit of 0.1 ng/mm2 of protein (BSA) and an extremely low background, ProteoSilver leads to superior detection of low abundance proteins.

Application

ProteoSilver Silver Stain Kit has been used for staining of the gels for visualizing proteins.

Features and Benefits

  • Premixed and preweighed solutions reduce time and cost of purchasing and preparing individual components
  • Optimized protocols help establish conditions for best results
  • High sensitivity and low background ensure very low abundance proteins can be detected and resolved from other proteins
  • MALDI Compatible - Protein spots of interest can be further characterized by mass spectrometry
  • Room Temperature Stability allows easy and convenient storage

Legal Information

ProteoSilver is a trademark of Sigma-Aldrich Co. LLC

Signal Word

Danger

Hazard Classifications

Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 1B - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Muta. 2 - Ox. Liq. 2 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

5.1B - Oxidizing hazardous materials

WGK

WGK 3

Flash Point(F)

132.8 °F - closed cup

Flash Point(C)

56 °C - closed cup

Certificate of Analysis

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Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Jean-Paul Lasserre et al.
Electrophoresis, 27(16), 3306-3321 (2006-07-22)
Study of the complexome - all the protein complexes of the cell - is essential for a better understanding and more global vision of cell function. Using two-dimensional blue native/SDS-PAGE (2-D BN/SDS-PAGE) technology, the cytosolic and membrane protein complexes of
F Gharahdaghi et al.
Electrophoresis, 20(3), 601-605 (1999-04-27)
Mass spectrometry is a powerful technique for the identification of proteins at nanogram quantities. However, some degree of sample preparation prior to mass spectrometry is required, and silver-stained protein gel samples are most problematic. Here we report our strategy to
Yong-Feng Han et al.
Cell research, 24(12), 1445-1465 (2014-11-26)
The SU(VAR)3-9-like histone methyltransferases usually catalyze repressive histone H3K9 methylation and are involved in transcriptional gene silencing in eukaryotic organisms. We identified a putative SU(VAR)3-9-like histone methyltransferase SUVR2 by a forward genetic screen and demonstrated that it is involved in

Protocols

Hand-casting gels for PAGE and SDS-PAGE using TurboMix™ Bis-Tris Gel Casting Kits

Overview of polyacrylamide gel chemistry and detailed instructions for hand-casting polyacrylamide gels using TurboMix™ Bis-Tris Gel Casting Kits

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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