M9274

Sigma-Aldrich

Murashige and Skoog Basal Medium

suitable for plant cell culture, with sucrose and agar

Synonym(s):
MS Basal Medium
MDL number:
NACRES:
NA.72

Quality Level

form

powder

application(s)

cell culture | plant: suitable

Featured Industry

Agriculture

shipped in

ambient

storage temp.

2-8°C

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Application

Murashige and Skoog Basal Medium has been used for culturing Arabidopsis transgenic seeds for cotyledon development, pearl millet (Pennisetum glaucum), and Rosmarinus ofcinalis L. for callus induction

Formula variant

With the macro- and micronutrients, vitamins, sucrose and agar as described by Murashige and Skoog (1962).

Media Formulation

Quantity

Formulated to contain 42.4 grams of powder per liter of medium.

Pictograms

Flame over circleExclamation mark

Signal Word

Warning

Hazard Statements

RIDADR

UN 1477 5.1 / PGII

WGK Germany

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How firm is the gel in the final sterile medium preparation of Murashige and Skoog Basal Medium, Product M9274?

    This formulation contains 8g/L of standard agar resulting in fairly firm and rigid culture medium.

Laure Audonnet et al.
Gene expression patterns : GEP, 25-26, 1-7 (2017-04-13)
Dimethylation of histone H3 lysine 9 (H3K9me2) is a heterochromatic mark linked to DNA methylation and gene repression. Removal of H3K9me2 from gene bodies by the jmjC histone demethylase IBM1/JMJ25 inhibits DNA methylation and derepresses gene expression. In this work...
Antoine Fort et al.
The New phytologist, 209(2), 590-599 (2015-09-24)
Heterosis is the phenomenon whereby hybrid offspring of genetically divergent parents display superior characteristics compared with their parents. Although hybridity and polyploidy can influence heterosis in hybrid plants, the differential contributions of hybridity vs polyploidy to heterosis effects remain unknown....
K Sopalun et al.
Cryo letters, 31(4), 347-357 (2010-09-08)
Three vitrification-based methods for the cryopreservation of Grammatophyllum speciosum protocorms were invesigated: droplet-vitrification, encapsulation-dehydration and encapsulation-vitrification. Protocorms, 0.1 cm in diameter, developed from 2-month-old germinating seeds were used. For droplet-vitrification, protocorms were precultured on filter paper soaked in half strength...
Iram Siddique et al.
Applied biochemistry and biotechnology, 162(7), 2067-2074 (2010-05-13)
An effective protocol was developed for in vitro regeneration of the Cassia angustifolia via indirect organogenesis from petiole explants excised from 21-day-old axenic seedlings. Organogenic callus were induced on Murashige and Skoog (MS) medium supplemented with 5.0 µM 2,4-dichlorophenoxy acetic acid...
C Srinivasan et al.
Plant cell reports, 30(4), 655-664 (2011-01-08)
Transgenic plants of tobacco (Nicotiana tabacum L) and European plum (Prunus domestica L) were produced by transforming with the apple class 1 KNOX genes (MdKN1 and MdKN2) or corn KNOX1 gene. Transgenic tobacco plants were regenerated in vitro from transformed...

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