Agar contains agarose and agaropectin, with agarose being the major gelling heteropolysaccharide. Agarose contains β-d-galactose and 3,6-anhydro-α-l-galactose, linked by glycosidic bonds β(1-4) called the neoagarobiose. It also has agarobiose with same disaccharide units with α(1-3) linkage. The major source of agar is the cell wall of seaweed from Rhodophyceae family.
Agar has been used as a component of EDMm (modified version of Eucalyptus dunnii basal medium (EDM)) for development of shoots of Eucalyptus dunnii clones. It has also been used as a component of Murashige and Skoog medium for in vitro culture of Strain TC09 of Cladosporium sphaerospermum.
Agar has been used as a component
- of Gamborg′s B5 regeneration medium
- of Murashige and skoog (MS) medium for the germination of Hypericum triquetrifolium Turra seeds
- in shoot inducing and naphthalene acetic acid pretreatment medium for the regeneration of Arabidopsis thaliana seedlings
100, 500 g in poly bottle
1 kg in poly bottle
2.5 kg in poly drum
Agar is majorly used as microbial solid culture media. It is used in the range of 0.5 to 1.0% (w/v) to solidify plant tissue culture media. It is essential for in vitro root development.
Purified for research and protoplast culture.
Typical working concentration: 6-12 g/L.