M6774

Sigma-Aldrich

McCown′s Woody Plant Basal Salt Mixture

powder, suitable for plant cell culture

NACRES:
NA.72

form

powder

Quality Level

application(s)

cell culture | plant: suitable

Featured Industry

Agriculture

storage temp.

2-8°C

General description

McCown′s Woody Plant Basal Salt Mixture is the non-vitamin component (chemstock) of the Woody Plant Medium (WPM) is used for propagation and elongation of root cultures.

Application

McCown′s Woody Plant Basal Salt Mixture has been used to culture apical and axillary buds. It has also been used to subculture individual shoots for the establishment of shoot tips in tissue culture.

Formula variant

With the macro- and micronutrients as described by Lloyd and McCown (1981).

Media Formulation

Quantity

Formulated to contain 2.3 grams of powder per liter of medium.

Pictograms

Flame over circleCorrosion

Signal Word

Danger

Hazard Statements

hazcat

Eye Dam. 1 - Ox. Sol. 3

storage_class_code

5.1B - Oxidizing hazardous materials

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Commercially-feasible micropropagation of Mountain Laurel, Kalmia latifolia, by shoot tip culture.
Lloyd, G., and McCown, B.H.
Proc. Int. Plant Prop. Soc., 30, 421-427 (1981)
Enhanced micropropagation and establishment of grapevine rootstock genotypes
Alzubi H, et al.
International Journal of Plant Developmental Biology, 6(1), 9-14 (2012)
Guo-Qing Song et al.
Plant cell reports, 25(2), 117-123 (2005-12-22)
Sour cherry (Prunus cerasus L.) scion cv. Montmorency and rootstock cv. Gisela 6 (P. cerasus x P. canescens) were transformed using Agrobacterium tumefaciens strain EHA105:pBISN1 carrying the neomycin phosphotransferase gene (nptII) and an intron interrupted ss-glucuronidase (GUS) reporter gene (gusA)....
Ercan Arican et al.
Journal of environmental biology, 29(6), 841-844 (2009-03-21)
This study was conducted to explain difficulties of indirect regeneration of forest trees in tissue culture conditions. For this purpose, changes of antioxidant defense system enzymes; superoxide dismutase (SOD), peroxidase (POD) activities were determined during calli formation on young apical...
Siriwan Phongprueksapattana et al.
Zeitschrift fur Naturforschung. C, Journal of biosciences, 63(9-10), 691-698 (2008-12-02)
Hairy root cultures of Mitragyna speciosa were established by infection of Agrobacterium rhizogenes ATCC 15834 and maintained in McCown woody plant medium (WPM) supplemented with 0.5 mg/1 naphthaleneacetic acid. The hairy roots were identified for the rooting genes loci of...
Articles
Classical plant tissue culture media developed years ago by pioneers such as Murashige, Skoog, Gamborg, and others still play a vital role in plant tissue culture research today.
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