MilliporeSigma
All Photos(2)

E0282

Sigma-Aldrich

MaxGel ECM

mixture, liquid

Synonym(s):
Human Basement Membrane Extract (BME)
eCl@ss:
32190102
NACRES:
NA.71

Quality Level

form

liquid

storage temp.

−70°C

Looking for similar products? Visit Product Comparison Guide

Application

MaxGel ECM is used for the following applications:
  • Extracellular matrix (ECM) protein binding assays
  • Cell invasion assay
  • Cell culture
This product creates a three-dimensional environment that improves cellular proflieration by promoting cell growth and migration, and has been shown to aid the proliferation of many types of cells, including human embryonic stem cells, neural stem cells, neurons, glia, astrocytes, fibroblasts, hepatocytes, and keratinocytes.

Components

MaxGel ECM contains human extracellular matrix components including collagens, laminin, fibronectin, tenascin, elastin and a number of proteoglycans and glycosaminoglycans. This basement membrane extract reproduces the cooperative interactions between epithelia and mesenchyme that occur during the development and organotypic cell culture of skin.

Caution

MaxGel will remain stable for at least 1 year when stored at -70°C.

Legal Information

MaxGel is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Quotes and Ordering

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. Will Product E0282, MaxGel ECM, work for adherent cells and suspension cells?

    Yes, this product works for both cell types.

  6. How is Product E0282, MaxGel ECM, supplied?

    E0282 is supplied at a concentration of 1 mg/ml in DMEM. 

  7. What is an appropriate working concentration for Product E0282, MaxGel ECM?

    MaxGel works best undiluted or even diluted 1:2-1:4.  For a thin coat plating method, this material can be diluted as high as 1:100. 

  8. How do I handle Product E0282, MaxGel ECM?

    Remove the product from the freezer and thaw on ice just prior to use. It quickly forms a gel when allowed to warm to room temperature, so it is necessary to keep the product on ice until coating.

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

J Křivánek et al.
Folia biologica, 60 Suppl 1, 30-38 (2014-11-05)
Neural crest cells (NCCs) derive early in vertebrate ontogenesis from neural tube as a population of migratory cells with exquisite differentiation potential. Abnormalities in NCC behaviour are cause of debilitating diseases including cancers and a spectrum of neurocristopathies. Thanks to
Pyrimido[4,5-d]pyrimidin-4(1H)-one derivatives as selective inhibitors of EGFR threonine790 to methionine790 (T790M) mutants.
Tianfeng Xu et al.
Angewandte Chemie (International ed. in English), 52(32), 8387-8390 (2013-06-28)
H M Sharon Goh et al.
Applied and environmental microbiology, 79(21), 6535-6543 (2013-08-21)
Acinetobacter baumannii is a multidrug-resistant pathogen associated with hospital outbreaks of infection across the globe, particularly in the intensive care unit. The ability of A. baumannii to survive in the hospital environment for long periods is linked to antibiotic resistance
Kang Li et al.
International journal of clinical and experimental pathology, 8(9), 10941-10946 (2015-12-01)
This study aims to explore the effects of vein subvolution combined with neural stem cells on nerve regeneration. Animal model of sciatic nerve defects was established with SD rats. A total of 63 SD rats were divided into control group
Bin He et al.
Oncology reports, 29(3), 1115-1123 (2012-12-21)
LIM and SH3 protein 1 (LASP1) is an actin-binding protein which is overexpressed in many types of cancers and plays important roles in cancer progression. however, the role of LASP1 in esophageal squamous cell carcinoma (ESCC) is still unknown. We sought

Articles

MaxGel™ ECM Hydrogel: An In Vitro Human Cell Derived Basement Membrane Extract for 3D Cell Culture Applications

Human cell derived basement membrane extract (BME) hydrogel for 3D cell culture of primary cell, stem cell and xenograph cancer models.

Hydrogels for 3D cell culture

Hydrogela are the most widely used systems for 3D cell culture. Learn more about this technology (what are hydrogels? How to chose?)

3D Cell Culture Tools and Techniques

3D cell culture overview. Learn about 2D vs 3D cell culture, advantages of 3D cell culture, and techniques available to develop 3D cell models

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service