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B3795

Sigma-Aldrich

Brain-derived neurotrophic factor human

BDNF, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Synonym(s):
BDNF, Abrineurin
MDL number:
NACRES:
NA.77

Quality Level

biological source

human

recombinant

expressed in E. coli

assay

≥95% (SDS-PAGE)

form

lyophilized powder

potency

≤15 ng/mL ED50

quality

endotoxin tested

mol wt

~27 kDa

packaging

pkg of 10 μg
pkg of 5 μg

storage condition

avoid repeated freeze/thaw cycles (Do not store in a frost-free freezer.)

technique(s)

cell culture | mammalian: suitable

impurities

≤1.00 EU/μg Endotoxin level

UniProt accession no.

storage temp.

−20°C

Gene Information

General description

BDNF is a member of the neurotrophin family of growth factors that includes NGF, NT-3, and NT-4. All neurotrophins have six conserved cysteine residues and share a 55% sequence identity at the amino acid level. BDNF has been shown to enhance the survival and differentiation of several classes of neurons in vitro, including neural crest and placode-derived sensory neurons, dopaminergic neurons in the substantia nigra, basal forebrain cholinergic neurons, hippocampal neurons, and retinal ganglial cells.1 BDNF is expressed within peripheral ganglia and is not restricted to neuronal target fields, raising the possibility that BDNF has paracrine or even autocrine actions on neurons as well as non-neuronal cells.
The active form of BDNF recombinant human protein (27 kDa) is a dimer formed by two identical 119 amino acid subunits held together by strong hydrophobic interactions

Application

Brain-derived neurotrophic factor (BDNF) recombinant human protein has been used as a supplement in various types of culture media.

Biochem/physiol Actions

Brain-derived neurotrophic factor (BDNF) is a 13.6 kDa (or 27.2 kDa dimer) member of the neurotrophin family. BDNF has identical amino acid sequence in human, mouse, and pig with full cross-reactivities. BDNF is important in development and maintenance of neuronal populations within the central nervous system or cells directly associated with it. BDNF has been shown to enhance the survival and differentiation of several classes of neurons in vitro, including neural crest and placode-derived sensory neurons, dopaminergic neurons in the substantia nigra, basal forebrain cholinergic neurons, hippocampal neurons, and retinal ganglial cells.

Physical form

lyophilized from a sterile 0.2 μm filtered solution with BSA

Analysis Note

The biological activity is determined by its ability to stimulate the proliferation of human neuroblastoma cells, SH-SY5Y.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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