All Photos(1)



ECM Gel from Engelbreth-Holm-Swarm murine sarcoma

growth-factor reduced, without phenol red, liquid, BioReagent, suitable for cell culture

GFR Basement Membrane Extract (BME)
MDL number:

Quality Level

product line





7-9 mg/mL


cell culture | mammalian: suitable

shipped in

dry ice

storage temp.


Looking for similar products? Visit Product Comparison Guide


ECM Gel from Engelbreth-Holm-Swarm murine sarcoma has been used to coat tissue culture plates for different glioma subtypes and C2C12 muscle cells. It has also been used as a scaffold to increase myotube attachment, maturation and survival.
ECM gel can be used with epithelial cells, endothelial cells, muscle cells, nerve cells and tumor cells.

Biochem/physiol Actions

The ECM gel will undergo thermally activated polymerization when brought to 20-40°C, forming a reconstituted basement membrane. PC12 cells will show neurite formation within 2 days when they are grown on a thin layer of this ECM gel.


ECM was prepared to a protein concentration of 8-12 mg/mL, containing laminin as a major component, collagen type IV, heparin sulfate proteoglycan, entactin, and other minor components. Addition of collagen type IV to the ECM gel increases polymerization.
The growth factor reduction modification allows for more control of medium, better interpretation of results and increased polymerization. The medium also lacks phenol red, which can interfere with some analyses.


For long term storage, keep product at -20°C. The ECM gel may be stored at 2-8°C for up to 72 hours.

Preparation Note

This product is prepared from Engelbreth-Holm-Swarm sarcoma in mice; dialyzed against chloroform. Gel should be thawed overnight at 2-8°C before use and dispensed to a multiwall plate using a plate and pipettes that are pre-cooled to 2-8°C. The gel may contain precipitates which do not influence its activity. The gel may also be diluted up to twofold with 2-8°C Dulbecco′s Modified Eagle′s Medium, and should be done before gel is added to the plate. The product will gel within 5 minutes at 20°C. For prolonged manipulations, work should be conducted below 10°C. Cells can be plated on top of a thin gel layer of 0.5 mm or cultured inside a 1 mm layer. If you culture cells inside, add the cells to the gel prior to plating at a recommended density of 3-4 x 104 cells per mL. To dissociate cells from this gel, use protease in PBS without calcium, magnesium, or EDTA, at a concentration of 0.6-2.4 units per mL.

Storage Class Code

10 - Combustible liquids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Product Information Sheet

Quotes and Ordering

  1. How does the storage temperature relate to shipping conditions?

    The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment. See Shipping and Storage for more information.

  2. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  3. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  4. Are ECM Gels, Products E1270 and E6909, the same as Matrigel (from BD)?

    Product No. E1270, ECM gel is the equivalent of Matrigel from BD only produced by Sigma-Aldrich.

  5. Are Products E1270 and E6909, ECM Gel from Engelbreth-Holm-Swarm murine sarcoma, growth factor reduced like Matrigel (from BD)?

    Product No. E1270 is not growth factor reduced. Product No. E6909 is ECM gel, growth factor reduced.

  6. Do Products E1270 and E6909, ECM Gel from Engelbreth-Holm-Swarm murine sarcoma, contain phenol red?

    Product E1270 ECM gel does contain phenol-red from the Dulbecco's modified Eagle's medium component of the product.  Product No. E6909, ECM gel, growth factor reduced, is phenol red free.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Muscle Cell and Tissue: Current Status of Research Field (2018)
Immunohistochemical Characterization and Sensitivity to Human Adenovirus Serotypes 3, 5, and 11p of New Cell Lines Derived from Human Diffuse Grade II to IV Gliomas
Niittykoski M, et al.
Translational Oncology, 10(5), 772-779 (2017)
Lampson M Fan et al.
The Journal of clinical investigation, 129(8), 3374-3386 (2019-07-23)
Oxidative stress plays an important role in aging-related neurodegeneration. This study used littermates of WT and Nox2-knockout (Nox2KO) mice plus endothelial cell-specific human Nox2 overexpression-transgenic (HuNox2Tg) mice to investigate Nox2-derived ROS in brain aging. Compared with young WT mice (3-4
Gesa Volkers et al.
ACS chemical biology, 13(8), 2320-2328 (2018-07-18)
Polysialyltransferases synthesize polysialic acid on cell surface-expressed glycoconjugates, which is crucial for developing processes and signaling pathways in eukaryotes. Recent advances in cancer research have rendered polysialyltransferases important drug targets because polysialic acid contributes to cancer cell progression, metastasis, and
Bhakti R Pathak et al.
Molecular and cellular biochemistry, 411(1-2), 11-21 (2015-09-16)
Cysteine-rich secretory protein 3 (CRISP-3) is upregulated in prostate cancer as compared to the normal prostate tissue. Higher expression of CRISP-3 has been linked to poor prognosis and hence it has been thought to act as a prognostic marker for


ECM Gel Matrix: Protocols Using EHS Basement Membrane Extracts

Cell culture protocols using Sigma ECM Gel Matrix EHS basement membrane extract (BME) for neurite outgrowth, cell invasion and angiogenesis tube formation assays.

Attachment Factors for 3-Dimensional Cell Culture

Attachment Factors for 3-Dimensional Cell Culture

Angiogenesis Assays

Cell based angiogenesis assays to analyze new blood vessel formation for applications of cancer research, tissue regeneration and vascular biology.

Hydrogels for 3D cell culture

Hydrogela are the most widely used systems for 3D cell culture. Learn more about this technology (what are hydrogels? How to chose?)

See All

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service