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TRUE1

Sigma-Aldrich

TrueGel3D Hydrogel Kits

CD cell-degradable crosslinker and RGD peptide

shipped in

dry ice

storage temp.

−70°C

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General description

The TrueGel3D hydrogel pre-configured kit is used to prepare a chemically defined hydrogel with stiffness of ~400 Pa (shear modulus) that can be customized to match that of native cell environment. The TrueGel3D hydrogel is formed by crosslinking of RGD (arginylglycylaspartic acid) degradable polymer with CD cell-degradable crosslinker. The cells are encapsulated during crosslinking, where they can adhere to the polymer through the RGD peptide and grow within the hydrogel. The CD cell-degradable crosslinker is composed of matrix metalloprotease (MMP)-cleavable peptide (Pro-Leu-Gly-Leu-Trp-Ala), which allows cells to spread and migrate by secreting matrix metalloproteases (MMP1, MMP3, MMP7 and MMP9).

Gel can be further dissolved by TrueGel3D Enzymatic Cell Recovery Solution (TRUEENZ-500UL) for post-culture analysis including RT-PCR, western blots and other molecular biological studies.

Application

Suitable for 3D-culture of various cell types, spheroids and tissue samples. It has been used for cell migration and invasion studies, co-culture experiments (tumor cells with fibroblasts) and cell growth and differentiation studies.

Features and Benefits

  • Easy to use and pre-configured
  • Gel with soft stiffness of 400 Pa
  • Contains RGD degradable polymer for cell attachment
  • Allow cell recovery and post-culture analyses

Components

TRUE1-1KT contains lyophilized RGD degradable polymer and CD cell-degradable crosslinker with reconstitution buffer and water.

Caution

Avoid prolong exposure of CD cell-degradable crosslinker to air.

Storage Class Code

10 - Combustible liquids

Certificate of Analysis

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Certificate of Origin

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Product Information Sheet

Quotes and Ordering

Articles

Hydrogels for 3D cell culture

Hydrogela are the most widely used systems for 3D cell culture. Learn more about this technology (what are hydrogels? How to chose?)

True Gel3D™ Hydrogel Troubleshooting Guide

TrueGel3D hydrogels kits for 3D cell culture troubleshooting guide

TrueGel3D™ Hydrogel for 3D Cell Culture

TrueGel3D™ is a synthetic hydrogels platform for 3D cell culture that aim to mimic the natural extracellular environment for more physiological culture conditions

Protocols

TrueGel3D™ hydrogel CD cell-degradable crosslinker and RGD peptide (TRUE1) preparation protocol

A specific protocol for TrueGel3D™ hydrogel CD cell-degradable crosslinker and RGD peptide (TRUE1) kits for 3D cell culture is available

TrueGel3D™ Fast Gelling Hydrogels Preparation Protocol

TrueGel3D™ is a chemically-defined hydrogel formed by mixing polymers with crosslinkers. This hydrogel permits the encapsulation of cells to put them in a three-dimensional environment to better mimic the native tissue environment.

Related Content

Advanced Protocol: How to Customize your TrueGel3D™ Hydrogel

An advanced protocol is available for TrueGel 3D kits for 3D cell culture for peptides concentration or stiffness optimisation

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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