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TRUE9

Sigma-Aldrich

TrueGel3D Hydrogel Kits

SLO-PVA, PEG non cell-degradable crosslinker

shipped in

dry ice

storage temp.

−70°C

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General description

The gel is formed by stable thioether bonds between thiol groups on PEG non cell-degradable crosslinker and thio-reactive groups on SLO-PVA. The polymer, SLO-PVA is functionalized with thiol-reactive groups which react with crosslinker at slower rate than maleimides in FAST-PVA. SLO-PVA does not have sites for cell attachment, but it can be customized to conjugate RGD peptide using TrueGel3D RGD peptide (TRUERGD-1EA) prior to the crosslinking step.

Application

TRUE9-1KT is used to generate hydrogels of different stiffness (0.15 to 5 KPa shear modulus) to mimic in-vivo conditions and to investigate the effect of matrix stiffness on cell physiology and behavior.SLO-PVA based gels are used to grow cells that do not need attachment to hydrogel for survival but rather use their secreted extracellular matrix. The SLO-PVA based hydrogels are preferred over dextran-based gels for long-term culture of cells producing carbohydrate degrading enzymes. TrueGel3DTM Hydrogel Kits has been used in the generation of 3D gel based tumor models.

Features and Benefits

  • Slow gelling (takes 6 minutes to 25 minutes, depends on gel stiffness)
  • Flexibility in modulating gel stiffness
  • Does not allow cell migration
  • Does not allow cell recovery and post-culture analyses
  • Cell inert hydrogel (if not customized with RGD peptide)

Components

TRUE9-1KT contains SLO-PVA solution in phosphate buffer and lyophilized PEG non cell-degradable crosslinker with reconstitution buffer and water.

Caution

Avoid prolong exposure of PEG non cell-degradable crosslinker to air and store SLO-PVA in small portions to avoid frequent freeze-thaw cycles.

Pictograms

Corrosion

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2

Storage Class Code

8A - Combustible, corrosive hazardous materials

Certificate of Analysis

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Certificate of Origin

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Product Information Sheet

Quotes and Ordering

Design of Spherically Structured 3D In vitro Tumor Models-Advances and Prospects
Ferreira LP, et al.
Acta Biomaterialia (2018)

Articles

Hydrogels for 3D cell culture

Hydrogela are the most widely used systems for 3D cell culture. Learn more about this technology (what are hydrogels? How to chose?)

True Gel3D™ Hydrogel Troubleshooting Guide

TrueGel3D hydrogels kits for 3D cell culture troubleshooting guide

TrueGel3D™ Hydrogel for 3D Cell Culture

TrueGel3D™ is a synthetic hydrogels platform for 3D cell culture that aim to mimic the natural extracellular environment for more physiological culture conditions

Protocols

TrueGel3D™ Slow Gelling Hydrogels Preparation Protocol

TrueGel3D™ is chemically defined hydrogel, enables cell growth in 3D environment and mimics native tissue environment. Slow gelation facilitates filling of microchannels or syringes. Find detail protocol of slow gelation for TrueGel3D™ kits

Related Content

Advanced Protocol: How to Customize your TrueGel3D™ Hydrogel

An advanced protocol is available for TrueGel 3D kits for 3D cell culture for peptides concentration or stiffness optimisation

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