MilliporeSigma
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HYSHP020

Sigma-Aldrich

HyStem®-HP Cell Culture Scaffold Kit

For 7.5 mL of hydrogel scaffold solution

NACRES:
NA.75

Quality Level

storage temp.

−20°C

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General description

The HyStem®-HP Cell Culture Scaffold Kit provides an excellent starting point for optimizing the matrix for stem cell culture, where slowly released growth factors are crucial in re-creating a stem cell niche. To affect specific cell performance, growth factors or ECM proteins may be added to the HyStem-HP hydrogel. Unlike animal-derived ECM products, this kit contains three fully chemically defined components, which are nonimmunogenic:
  • HyStem®-HP – a thiol-modified hyaluronan (a major constituent of native ECM), carboxymethyl hyaluronic acid-thiopropanoyl hydrazide (CMHA-S, CMHA-DTPH, carboxymethyl hyaluronic acid-DTPH) with heparinthiopropanoyl hydrazide (HP-DTPH). HyStem-HP (CMHA-DTPH with HP-DTPH) contains 99.7 wt% CMHA-DTPH and 0.3 wt% HP-DTPH.
  • Gelin-S – a thiol-modified gelatin (denatured collagen), carboxymethyl gelatin-thiopropanoyl hydrazide (GTN-DTPH, carboxymethyl gelatin-DTPH)
  • Extralink® – a thiol-reactive crosslinker, polyethylene glycol diacrylate (MW = 3,400 g/mole, PEGDA)

Features and Benefits

Hystem-HP enables ionic binding and slow release of growth factors through thiolated heparin; growth factors can be integrated via the matrix rather than cell culture medium. Hystem-HP also enables the attachment of a wide variety of cell types through Gelin-S (thiolated gelatin).

With Hystem-HP, researchers can tailor the incorporation of ECM proteins and growth factors as well as the elasticity of the hydrogel and cell incorporation (encapsulations or top plating) to create the specific 3D environment required by their cells.

Hystem-HP is recommended for both in vivo and in vitro experimentation.
Customizable, well characterized and consistent, Hystem-HP hydrogel cell culture scaffolds provide a complex, three dimensional environment in which cells are able to proliferate, much as they would in vivo.
  • Customizable - each distinct cell type in a multicellular organism has its own unique microenvironment. Hystem-HP offers the flexibility to tailor the environments of cultured cells to mimic their in vivo counterparts and optimize proliferation and differentiation.
  • Synthetic - As a synthetic matrix, Hystem-HP offers greater control of the cellular environment than ambiguous extracted ECM alternatives. All components are well characterized and consistently formulated. Hystem-HP contains Hyaluronic acid (synthetic), Extralink (synthetic), Gelin-S (porcine), Heparin and Water.
  • Biologically accurate - Rich in hyaluronic acid and denatured collagen, the Hystem-HP hydrogel closely mimics the complex three dimensional cellular environments found in life, providing an optimal environment for culturing your cells.

Legal Information

Extralink is a registered trademark of Glycosan BioSystems, Inc.
Gelin is a trademark of Biopharm Ltd.
Hystem is a registered trademark of BIOTIME, INC

Pictograms

CorrosionExclamation mark

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Eye Dam. 1 - Skin Irrit. 2 - Skin Sens. 1

Storage Class Code

12 - Non Combustible Liquids

Certificate of Analysis

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Certificate of Origin

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Product Information Sheet

Quotes and Ordering

Articles

What is a Cell Delivery Vehicle?

Cell delivery vehicle are components that can be associated with cells and that allow transplantation in human or animals hosts. These can be natural or synthetic components that will form a gel, embedding the cells.

Heparin Versus Heparin Sulfate Proteoglycans

Heparin is distinct from heparan sulfate proteoglycans (HS) which is primarily produced by mast cells. While, HS is produced by all cell types. Know more about the difference between heparin and heparin sulfate proteoglycan and select the right component for your study.

What is Hyaluronan?

Hyaluronan is a major component of the extracellular matrix. It’s the simplest glycosaminoglycan, and it plays several biological roles.

HyStem™ Hyaluronic Acid Based Hydrogels for 3D Cell Culture Applications

Hystem 3D hydrogels are based on hyaluronic acid, a major component of the extracellular matrix. 3D cell culture can be done using Hystem hydrogels.

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