CMC0001

SIG10 Chemically Competent Cells

for protein expression and DNA plasmid production

• NACRES: NA.85

Properties

• grade: for molecular biology
• form: buffered aqueous solution
• cell transformation: competent cell type: chemically competent; transformation efficiency: ≥1 × 10⁹ cfu/μg
• shipped in: dry ice
• storage temp.: −70°C

Description

General description

The SIG10 Chemically Competent Cells are a derivative of Escherichia coli that have been optimized for high transformation efficiency.
These cells are ideal for cloning and propagation of plasmid, cosmid, or fosmid clones and are highly efficient for routine cloning applications.
They share the most useful genetic elements of standard cloning strains like DH5α^™ DH10B^™, JM109, TOP10, etc. and directly replace them in cloning protocols.
They are are provided in 40 μL, 80 μL and 160 μL aliquots, each being sufficient for one, two and four transformations respectively.
The cells have a transformation efficiency of >1 x 10⁹ cfu/μg
The 96-well format are provided in aliquots of 20 μL per well and have a transformation efficiency of >1 x 10⁸ cfu/μg.

Genotype
F- mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara,leu)7697 galU galK rpsL nupG λ- tonA

Application

Suitable for bacterial transformations to recover high quality plasmid DNA

Features and Benefits

• share the most useful genetic elements of standard cloning strains like DH5α^™ DH10B^™, JM109, TOP10, etc. and directly replace them in cloning protocols.
• ensures recovery of stable and high quality plasmid DNA.
• high transformation efficiency
• provide the performance researchers need with ease of use.
• provide solutions for a wide range of applications at economical prices.
• Blue - white screening

Components

• SIG10 chemically competent cells
• pUC 19 transformation control DNA at 10 pg/μL
• recovery medium for cloning

Principle

The SIG10 cells contains the inactive mcr and mrr alleles, allowing methylated genomic DNA isolated directly from mammalian or plant cells to be cloned without deletions or rearrangements.
This strain also carries the recA1 and endA1 mutations. The recA1 genotype provides minimized recombination and aids in plasmid stability while endA1 provides for high quality plasmid DNA preparation.
They are bacteriophage T1-resistant (tonA mutation) and also resistant to streptomycin by virtue of rpsL mutation.

Legal Information

DH10B is a trademark of Life Technologies

DH5α is a trademark of Invitrogen Corp.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3