All Photos(3)



N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride


EDC, WSC hydrochloride, N-Ethyl-N′-(3-dimethylaminopropyl)carbodiimide hydrochloride, EDAC, EDC hydrochloride
Empirical Formula (Hill Notation):
C8H17N3 · HCl
CAS Number:
Molecular Weight:
EC Number:
MDL number:
PubChem Substance ID:

Quality Level



reaction suitability

reagent type: cross-linking reagent
reaction type: Peptide Synthesis


110-115 °C (lit.)


H2O: ≤100 mg/mL

storage temp.


SMILES string




InChI key


Looking for similar products? Visit Product Comparison Guide


N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride has been used:
  • for the immobilisation of trypsin onto self-assembled monolayers (SAMs)
  • as a component for the preparation of collagen matrices
  • for the preparation of phosphoethanolamine(PEt)-conjugated sepharose


1, 5 g in glass bottle

Biochem/physiol Actions

Water soluble condensing reagent. EDAC is generally utilized as a carboxyl activating agent for amide bonding with primary amines. In addition, it will react with phosphate groups. EDAC has been used in peptide synthesis; crosslinking proteins to nucleic acids; and preparation of immunoconjugates as examples. Typically, EDAC is utilized in the pH range 4.0-6.0 without buffers. In particular, amine and carboxylate buffers should be avoided.

Signal Word


Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2 Oral

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

The phosphocholine-binding pocket on C-reactive protein is necessary for initial protection of mice against pneumococcal infection
Gang TB, et al.
The Journal of Biological Chemistry, jbc-M112 (2012)
Development and characterization of a collagen-based matrix for vascularization and cell delivery.
Ellis CE, et al
BioResearch Open Access, 4(1), 188-197 (2015)
Yu-Chang Tyan et al.
Journal of materials science. Materials in medicine, 16(2), 135-142 (2005-03-04)
Self-assembled monolayers (SAMs) on coinage metal provide versatile modeling systems for studies of interfacial electron transfer, biological interactions, molecular recognition and other interfacial phenomena. Recently the bonding of enzyme to SAMs of alkanethiols onto Au electrode surfaces was exploited to
Sabine Hauptstein et al.
Journal of pharmaceutical sciences, 103(2), 594-601 (2014-01-03)
The objective of this study was to generate a water-soluble thiolated chitosan to enable the permeation-enhancing effect of chitosan at pH of at least 5.5 without losing the advantages of improved mucoadhesive properties. Therefore, the thiol-bearing polyoxyethylene ligand {O-(3-carboxylpropyl)-O'-[2-[3-mercaptopropionylamino)ethyl]-polyethyleneglycol} was
Jairo Pinto Oliveira et al.
Scientific reports, 9(1), 13859-13859 (2019-09-27)
Antibody-coated nanoparticles have recently attracted considerable attention, with the focus falling on diagnostics. Nevertheless, controlled antibody bioconjugation remains a challenge. Here, we present two strategies of bioconjugation with the aim of evaluating the best approach for the coupling of antibodies

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service