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E6383

Sigma-Aldrich

N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride

crystalline

Synonym(s):
EDC, WSC hydrochloride, N-Ethyl-N′-(3-dimethylaminopropyl)carbodiimide hydrochloride, EDAC, EDC hydrochloride
Empirical Formula (Hill Notation):
C8H17N3 · HCl
CAS Number:
Molecular Weight:
191.70
Beilstein:
5764110
EC Number:
MDL number:
PubChem Substance ID:

Quality Level

form

crystalline

reaction suitability

reagent type: cross-linking reagent
reaction type: Peptide Synthesis

mp

110-115 °C (lit.)

solubility

H2O: ≤100 mg/mL

storage temp.

−20°C

SMILES string

Cl.CCN=C=NCCCN(C)C

InChI

1S/C8H17N3.ClH/c1-4-9-8-10-6-5-7-11(2)3;/h4-7H2,1-3H3;1H

InChI key

FPQQSJJWHUJYPU-UHFFFAOYSA-N

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Application

N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride has been used:
  • for the immobilisation of trypsin onto self-assembled monolayers (SAMs)
  • as a component for the preparation of collagen matrices
  • for the preparation of phosphoethanolamine(PEt)-conjugated sepharose

Packaging

1, 5 g in glass bottle

Biochem/physiol Actions

Water soluble condensing reagent. EDAC is generally utilized as a carboxyl activating agent for amide bonding with primary amines. In addition, it will react with phosphate groups. EDAC has been used in peptide synthesis; crosslinking proteins to nucleic acids; and preparation of immunoconjugates as examples. Typically, EDAC is utilized in the pH range 4.0-6.0 without buffers. In particular, amine and carboxylate buffers should be avoided.

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2 Oral

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

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The Journal of Biological Chemistry, jbc-M112 (2012)
Development and characterization of a collagen-based matrix for vascularization and cell delivery.
Ellis CE, et al
BioResearch Open Access, 4(1), 188-197 (2015)
Yu-Chang Tyan et al.
Journal of materials science. Materials in medicine, 16(2), 135-142 (2005-03-04)
Self-assembled monolayers (SAMs) on coinage metal provide versatile modeling systems for studies of interfacial electron transfer, biological interactions, molecular recognition and other interfacial phenomena. Recently the bonding of enzyme to SAMs of alkanethiols onto Au electrode surfaces was exploited to
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Journal of pharmaceutical sciences, 103(2), 594-601 (2014-01-03)
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Jairo Pinto Oliveira et al.
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