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N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride

purum, ≥98.0% (AT)

EDC, WSC hydrochloride, N-Ethyl-N′-(3-dimethylaminopropyl)carbodiimide hydrochloride, EDAC, EDC hydrochloride
Empirical Formula (Hill Notation):
C8H17N3 · HCl
CAS Number:
Molecular Weight:
EC Number:
MDL number:
PubChem Substance ID:

Quality Level




≥98.0% (AT)




110-115 °C (lit.)
110-115 °C


H2O: soluble 1 gm/10 ml, clear to very slightly hazy, colorless to very faintly yellow

storage temp.


SMILES string




InChI key


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N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride has been used modify the cell surface of Escherichia coli to covalently couple substances. It has also been used as an activator to modify microfluidic chips to capture Escherichia coli.

Biochem/physiol Actions

N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide is a water soluble condensing reagent. EDAC is generally utilized as a carboxyl activating agent for amide bonding with primary amines. Additionally, it reacts with phosphate groups. It has been utilized in peptide synthesis, crosslinking proteins to nucleic acids as well as preparation of immunoconjugates.
Water soluble condensing reagent. EDAC is generally utilized as a carboxyl activating agent for amide bonding with primary amines. In addition, it will react with phosphate groups. EDAC has been used in peptide synthesis; crosslinking proteins to nucleic acids; and preparation of immunoconjugates as examples. Typically, EDAC is utilized in the pH range 4.0-6.0 without buffers. In particular, amine and carboxylate buffers should be avoided.

Signal Word


Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2 Oral

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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More Documents

Quotes and Ordering

Understanding bacterial uptake by protozoa: A versatile technique for surface modification of bacteria
Siegmund L and Wostemeyer J
Endocytobiosis and cell research, 27, 17-21 (2016)
Heather M Powell et al.
Biomaterials, 27(34), 5821-5827 (2006-08-22)
Collagen-based scaffolds are extensively utilized as an analog for the extracellular matrix in cultured skin substitutes (CSS). To improve the mechanical properties and degradation rates of collagen scaffolds, chemical cross-linking is commonly employed. In this study, freeze-dried collagen-GAG sponges were
Kunihiko Seno et al.
Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, 24(4), 505-508 (2008-04-12)
1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC.HCl) is a very useful agent to form amide bonds (peptide bonds) in an aqueous medium. A simple and fast detection system was developed using the reaction with pyridine and ethylenediamine in acidic aqueous solution and spectrophotometric flow
Laurie Broadwater et al.
Biochimica et biophysica acta, 1812(5), 630-641 (2011-02-08)
Mitochondrial dysfunction has been proposed to play a role in the neuropathology of multiple sclerosis (MS). Previously, we reported significant alterations in the transcription of nuclear-encoded electron transport chain genes in MS and confirmed translational alterations for components of Complexes
Ahu Arslan Yildiz et al.
Analytical biochemistry, 423(1), 39-45 (2012-02-07)
The analysis of membrane proteins is notoriously difficult because isolation and detergent-mediated reconstitution often results in compromising the protein structure and function. We introduce a novel strategy of combining a cell-free expression method for synthesis of a protein species coping


Carbodiimide-mediated peptide coupling

Carbodiimide-mediated peptide coupling remains to the most frequently used technique.

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