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N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride


EDC, WSC hydrochloride, N-Ethyl-N′-(3-dimethylaminopropyl)carbodiimide hydrochloride, EDAC, EDC hydrochloride
Empirical Formula (Hill Notation):
C8H17N3 · HCl
CAS Number:
Molecular Weight:
EC Number:
MDL number:
PubChem Substance ID:

Quality Level

product line



≤0.005% Phosphorus (P)
≤0.1% Insoluble matter

ign. residue



110-115 °C (lit.)


H2O: 0.5 M, clear to very slightly hazy, colorless to very faintly yellow

anion traces

sulfate (SO42-): ≤0.05%

cation traces

Al: ≤0.0005%
Ca: ≤0.0005%
Cu: ≤0.0005%
Fe: ≤0.0005%
K: ≤0.005%
Mg: ≤0.0005%
NH4+: ≤0.05%
Na: ≤0.005%
Pb: ≤0.001%
Zn: ≤0.0005%

storage temp.


SMILES string




InChI key


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N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride has been used:
  • for post-fixation and crosslinking of small ribonucleic acid (RNAs) species from formalin fixed paraffin embedded (FFPE) tissues, for fluorescent in situ hybridisation (FISH) and immunofluorescence (IF) signals
  • for cross-linking of nanofiber matrices with mouse lung extract
  • for activation of substrate XNA oligonucleotides


1, 5, 10, 25 g in poly bottle

Biochem/physiol Actions

Water soluble condensing reagent. EDAC is generally utilized as a carboxyl activating agent for amide bonding with primary amines. In addition, it will react with phosphate groups. EDAC has been used in peptide synthesis; crosslinking proteins to nucleic acids; and preparation of immunoconjugates as examples. Typically, EDAC is utilized in the pH range 4.0-6.0 without buffers. In particular, amine and carboxylate buffers should be avoided.

Signal Word


Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2 Oral

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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Chien-Chen Lin et al.
Biomaterials, 31(32), 8445-8453 (2010-08-17)
Monoclonal antibodies (mAbs) have been proven useful in research and clinical applications. However, the generation of mAbs by conventional hybridoma technology is time-, cost- and labor-consuming. Here we developed a simplified procedure for efficient generation and selection of antibody-producing hybridomas
Directed evolution of artificial enzymes (XNAzymes) from diverse repertoires of synthetic genetic polymers
Taylor AI and Holliger P
Nature Protocols, 10(10), 1625-1625 (2015)
Organ-derived coatings on electrospun nanofibers as ex vivo microenvironments
Fischer SN, et al.
Biomaterials, 32(2), 538-546 (2011)
Henry H Lee et al.
Nature communications, 10(1), 2383-2383 (2019-06-05)
DNA is an emerging medium for digital data and its adoption can be accelerated by synthesis processes specialized for storage applications. Here, we describe a de novo enzymatic synthesis strategy designed for data storage which harnesses the template-independent polymerase terminal
Hongyan Li et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 29(48), 15178-15186 (2009-12-04)
Electrical coupling of neurons is widespread throughout the CNS and is observed among retinal photoreceptors from essentially all vertebrates. Coupling dampens voltage noise in photoreceptors and rod-cone coupling provides a means for rod signals to enter the cone pathway, extending


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