335940

Xylene Cyanol FF

Dye content ≥75 %

• Synonym(s): Acid blue 147, Cyanol FF, XC, Xylene Cyanole FF
• Empirical Formula (Hill Notation): C₂₅H₂₇N₂NaO₆S₂
• CAS Number: 2650-17-1
• Molecular Weight: 538.61
• Colour Index Number: 42135
• EC Number: 220-167-5
• MDL number: MFCD00040657
• UNSPSC Code: 12171500
• PubChem Substance ID: 24860353
• NACRES: NA.47

Properties

• assay: ≥96.0% (HPLC)
• Quality Level: 200
• form: powder or crystals
• composition: Dye content, ≥75%
• mp: 295 °C (dec.) (lit.)
• solubility: water: 1 mg/mL, blue
• λmax: 615 nm
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: [Na+].CCNc1ccc(cc1C)C(=C2/C=C\C(=N/CC)C(C)=C2)\c3ccc(cc3S(O)(=O)=O)S([O-])(=O)=O
• InChI: 1S/C25H28N2O6S2.Na/c1-5-26-22-11-7-18(13-16(22)3)25(19-8-12-23(27-6-2)17(4)14-19)21-10-9-20(34(28,29)30)15-24(21)35(31,32)33;/h7-15,26H,5-6H2,1-4H3,(H,28,29,30)(H,31,32,33);/q;+1/p-1/b25-19-,27-23+;
• InChI key: VVLFAAMTGMGYBS-AHRQKMASSA-M

Description

General description

Xylene cyanol is often used as a tracking dye during agarose or polyacrylamide gel electrophoresis. It has a slightly negative charge and will migrate in the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The tracking dye typically migrates with the DNA molecules around 5kb. Xylene cyanol may also be used in loading buffer that facilitates the observation of the sample during gel loading and electrophoresis.

Safety Information

• pictograms: GHS07
• signalword: Warning
• hcodes: H319
• pcodes: P264 - P280 - P305 + P351 + P338 - P337 + P313
• Hazard Classifications: Eye Irrit. 2
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Gloves