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114391

Sigma-Aldrich

Bromophenol Blue

ACS reagent

Synonym(s):
3′,3″,5′,5″-Tetrabromophenolsulfonphthalein, Bromphenol Blue Sultone Form
Empirical Formula (Hill Notation):
C19H10Br4O5S
CAS Number:
Molecular Weight:
669.96
Beilstein:
61698
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

Quality Level

grade

ACS reagent

form

powder

technique(s)

titration: suitable

visual transition interval

3.0 - 4.6, yellow to blue

mp

273 °C (lit.)

solubility

ethanol: 0.1%

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

Oc1c(Br)cc(cc1Br)C2(OS(=O)(=O)c3ccccc23)c4cc(Br)c(O)c(Br)c4

InChI

1S/C19H10Br4O5S/c20-12-5-9(6-13(21)17(12)24)19(10-7-14(22)18(25)15(23)8-10)11-3-1-2-4-16(11)29(26,27)28-19/h1-8,24-25H

InChI key

UDSAIICHUKSCKT-UHFFFAOYSA-N

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Application

Bromphenol Blue is used as a tracking dye in DNA, RNA (agarose) and protein (polyacrylamide) gel electrophoresis. Bromophenol blue migrates at approximately the same rate as 300-500bp DNA in agarose gel and at the buffer front in protein polyacrylamide gels.

Packaging

5, 25 g in glass bottle

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Certificate of Origin

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Proceedings of the National Academy of Sciences of the United States of America, 111(39), E4076-E4085 (2014-09-18)
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The Journal of general virology, 96(Pt 3), 538-546 (2014-12-08)
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