All Photos(2)

O7252

Sigma-Aldrich

Orange G

certified by the Biological Stain Commission

Synonym(s):
Acid Orange 10, Wool Orange 2G, 7-Hydroxy-8-phenylazo-1,3-naphthalenedisulfonic acid disodium salt, 1-Phenylazo-2-naphthol-6,8-disulfonic acid disodium salt
Empirical Formula (Hill Notation):
C16H10N2Na2O7S2
CAS Number:
Molecular Weight:
452.37
Colour Index Number:
16230
Beilstein:
4120705
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

Quality Level

grade

certified by the Biological Stain Commission

form

powder

composition

Dye content, ≥80%

solubility

water: 1 mg/mL, clear

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[Na+].Oc1ccc2cc(cc(c2c1\N=N\c3ccccc3)S([O-])(=O)=O)S([O-])(=O)=O

InChI

1S/C16H12N2O7S2.2Na/c19-13-7-6-10-8-12(26(20,21)22)9-14(27(23,24)25)15(10)16(13)18-17-11-4-2-1-3-5-11;;/h1-9,19H,(H,20,21,22)(H,23,24,25);;/q;2*+1/p-2/b18-17+;;

InChI key

HSXUHWZMNJHFRV-QIKYXUGXSA-L

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General description

Orange G (C.l. 16230) is the disodium salt of benzeneazo-2-naphthol-6,8-disulfonic acid, C16H10N2O7S2Na2. Orange G, also known as wool orange 2G, is a component in several industry applications including cytology, histology, biology, and industrial staining. It is a component of Mallory′s connective tissue stain.

Application

Orange G is typically used as a background or cytoplasmic stain for various biological samples including a common application within clincial cytology within Papanicolaou varients.

Packaging

25, 100 g in glass bottle

Suitability

Certified for use in Mallory′s connective tissue stain; Wilson-Ezrin method for pituitary acidophils; Kreyberg′s method for keratin and mucus; as a counterstain following Heidenhain′s iron hematoxylin on paraffin sections; Flemming′s triple stain on paraffin sections of onion root.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Certificate of Origin

Integrity of chromatin and replicating DNA in nuclei released from fission yeast by semi-automated grinding in liquid nitrogen.
Givens RM
BMC Research Notes, 4, 499-499 (2011)
A R Khataee et al.
Journal of hazardous materials, 168(1), 451-457 (2009-03-13)
In order to discuss the effect of chemical structure on photocatalysis efficiency, the photocatalytic degradation of three commercial textile dyes (C.I. Acid Orange 10 (AO10), C.I. Acid Orange 12 (AO12) and C.I. Acid Orange 8 (AO8)) with different structure and
Chendong Shuang et al.
Water research, 46(14), 4417-4426 (2012-06-26)
In this paper, a novel quaternized magnetic resin, NDMP, was prepared and characterized. Two reactive dyes (RDs), Orange G (OG) and red RWO, were used as a small-molecular RD and large-molecular RD, respectively, to investigate their adsorption on NDMP. A
Honghai Wu et al.
Environmental technology, 33(13-15), 1545-1552 (2012-09-20)
Decolourization of the azo dye Orange G (OG) was investigated by using goethite/H2O2 as a heterogeneous Fenton-like reagent. Five principle operational parameters, namely pH, ion strength, concentrations ofgoethite (alpha-FeOOH) and hydrogen peroxide (H2O2), and reaction temperature, were taken into account
Gustavo A Blanco Heras et al.
Electrophoresis, 29(14), 3032-3037 (2008-07-26)
A method based on indirect photometric detection (IPD) in CE employing a blue LED (473 nm) as a light source and the highly absorbing (478 nm) anionic dye, Orange G, as the probe ion was developed for the sensitive analysis

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