1.15925

Orange G (C.I. 16230)

for microscopy Certistain^®

• Synonym(s): Orange G (C.I. 16230)
• Empirical Formula (Hill Notation): C₁₆H₁₀N₂Na₂O₇S₂
• CAS Number: 1936-15-8
• Molecular Weight: 452.37
• MDL number: MFCD00012457
• UNSPSC Code: 41116130
• EC Index Number: 217-705-6

Properties

• Quality Level: 400
• form: solid
• potency: >3000 mg/kg LD₅₀, oral (Rat)
• composition: Dye, ≥80% spectrophotometric assay
• pH: 9 (20 °C, 10 g/L in H₂O)
• solubility: 70 g/L
• bulk density: 430 kg/m³
• εmax: 380-500 at 476-481 nm in water at 0.015 g/L
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: 2-30°C
• InChI: 1S/C16H12N2O7S2.2Na/c19-13-7-6-10-8-12(26(20,21)22)9-14(27(23,24)25)15(10)16(13)18-17-11-4-2-1-3-5-11;;/h1-9,17H,(H,20,21,22)(H,23,24,25);;/q;2*+1/p-2/b18-16+;;
• InChI key: AEOVEGJBKQQFOP-DDVLFWKVSA-L

Description

General description

The dry dye Orange G (C.I. 16230) for microscopy Certistain^®, is used for the preparation of a staining solution for Papanicolaou′s staining in cytology in material of human origin. The effect of the Orange G staining solution is considerable in smears with keratinized cells under acidic pH conditions, when the obvious orangeophilic cytoplasmic stain is recognizable. As a Certistain^® dye it is chemically quality-certified according to highest standards and strict specifications. As an IVD product and CE registered, it can be used hassle-free in diagnostics and laboratory accreditations. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Analysis Note

Dye content (spectrophotometrically): ≥ 80 %
Identity
- sodium:
passes test
- UV/VIS-Spectrum: passes test
Absorption maximum λ max (water): 476 - 481 nm
Spec. Absorptivity A 1%/1cm (λ_max; 0.015 g/l; water): 380 - 500
TLC-Test: passes test
Loss on drying (110 °C): ≤ 15 %
Suitability for microscopy: passes test

Legal Information

CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_c: Not applicable