B8026

Bromophenol Blue sodium salt

for molecular biology, suitable for electrophoresis

• Synonym(s): 3′,3′′,5′,5′′-Tetrabromophenolsulfophthalein sodium salt
• Empirical Formula (Hill Notation): C₁₉H₉Br₄NaO₅S
• CAS Number: 34725-61-6
• Molecular Weight: 691.94
• Beilstein/REAXYS Number: 6995701
• EC Number: 252-170-2
• MDL number: MFCD00013793
• UNSPSC Code: 12171500
• PubChem Substance ID: 24892042
• NACRES: NA.47

Properties

• grade: for molecular biology
• Quality Level: 200
• form: powder
• technique(s): electrophoresis: suitable
• visual transition interval: 3.0-4.6, yellow to blue
• ε (extinction coefficient): ≥150000 at 597-603 nm in H₂O; ≥17000 at 250-256 nm in H₂O; ≥23000 at 306-312 nm in H₂O
• suitability: suitable for (Suitable For Use as a Tracking Dye For Electrophoresis in Alkaline and Neutral Buffers)
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: [Na+].Oc1c(Br)cc(cc1Br)C(=C2/C=C(Br)C(=O)C(Br)=C2)\c3ccccc3S([O-])(=O)=O
• InChI: 1S/C19H10Br4O5S.Na/c20-12-5-9(6-13(21)18(12)24)17(10-7-14(22)19(25)15(23)8-10)11-3-1-2-4-16(11)29(26,27)28;/h1-8,24H,(H,26,27,28);/q;+1/p-1
• InChI key: TWKDJMRHSLWRFI-UHFFFAOYSA-M

Description

General description

Bromophenol blue (BroB), an arylmethane color marker dye has a molecular mass of 670 Da.

Application

Bromophenol Blue sodium salt has been used in the buffer solution to differentiate dead and living cells. It has also been used as a constituent in gel loading buffer.

A tracking dye for nucleic acid or protein electrophoresis in agarose or polyacrylamide gels.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)