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252549

Sigma-Aldrich

Formaldehyde solution

ACS reagent, 37 wt. % in H2O, contains 10-15% Methanol as stabilizer (to prevent polymerization)

Synonym(s):
Formalin
Linear Formula:
HCHO
CAS Number:
Molecular Weight:
30.03
Beilstein:
1209228
MDL number:
PubChem Substance ID:
NACRES:
NA.21

grade

ACS reagent

Quality Level

vapor density

1.03 (vs air)

vapor pressure

52 mmHg ( 37 °C)
52 mmHg ( 37 °C)

assay

36.5-38.0%

form

liquid

autoignition temp.

572 °F

contains

10-15% Methanol as stabilizer (to prevent polymerization)

concentration

37 wt. % in H2O

impurities

≤0.006 meq/g Titr. acid

ign. residue

≤0.005%

color

APHA: ≤10

refractive index

n20/D 1.3765

density

1.09 g/mL at 25 °C (lit.)

anion traces

chloride (Cl-): ≤5 ppm
sulfate (SO42-): ≤0.002%

cation traces

Fe: ≤5 ppm
heavy metals: ≤5 ppm (by ICP-OES)

suitability

suitable for manufacturing use (Laboratory chemicals)

SMILES string

[H]C([H])=O

InChI

1S/CH2O/c1-2/h1H2

InChI key

WSFSSNUMVMOOMR-UHFFFAOYSA-N

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General description

Formaldehyde (formalin) is produced by oxidation of methanol. It is made of 37% formaldehyde and impurities such as methanol, small amounts of formic acid, aldehydes and ketones. It is used as a denaturant in formaldehyde-agarose gel electrophoresis of RNA.

Application

Formaldehyde solution has been used as a fixing agent to fix cells during immunofluorescence imaging and for cross-linking cells during chromatin immunoprecipitation (ChIP) assay.
Formaldehyde solution has been used for cross-linking/fixing of cells in ChIP (chromatin immunoprecipitation) assay. It has been used for fixing of cells for imaging.

Packaging

25, 100, 4×100, 500, 6×500 mL in glass bottle
1, 4 L in glass bottle

Physical form

This product is a solution of approximately 37% by weight of formaldehyde gas in water.

Other Notes

The article number 252549-4X2.5L will be discontinued. Please order the single bottle 252549-2.5L which is physically identical with the same exact specifications.
The article number 252549-6X1L will be discontinued. Please order the single bottle 252549-1L which is physically identical with the same exact specifications.

Signal Word

Danger

Hazard Classifications

Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 3 Oral - Carc. 1B - Eye Dam. 1 - Flam. Liq. 3 - Muta. 2 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 1 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

3 - Flammable liquids

WGK

WGK 3

Flash Point(F)

132.8 °F - closed cup

Flash Point(C)

56 °C - closed cup

Certificate of Analysis

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Certificate of Origin

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