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HT501128

Sigma-Aldrich

Formalin solution, neutral buffered, 10%

histological tissue fixative

MDL number:
PubChem Substance ID:
NACRES:
NA.47

Quality Level

biological source

synthetic

shelf life

Expiry date on the label.

concentration

10% formalin (approx. 4% formaldehyde)

application(s)

hematology: suitable
histology: suitable

color

colorless

pH

6.90-7.10

density

1.080 g/cm3

storage temp.

room temp

InChI

1S/CH2O/c1-2/h1H2

InChI key

WSFSSNUMVMOOMR-UHFFFAOYSA-N

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General description

10% Neutral buffered formalin (NBF) is the most commonly used fixative throughout the world for light microscopy and is a somewhat forgiving fixative. 10% NBF is well suited for large throughput laboratories, and requires a relatively short period of fixation, but can also be used for the long-term storage of tissue. Formalin fixation is thought to form between the aldehydes and the proteins, creating a gel, thus retaining cellular constituents in their in vivo relationship. Once properly fixed, the tissue should be able to withstand the subsequent stages of tissue processing or staining.

Application

Formalin solution, neutral buffered, 10% has been used as a popular general purpose histological fixative to fix tissues.
Formalin solution, neutral buffered, 10% has been used in oil-red-o staining for the differentiation of 3T3-L1 preadipocyte cell line and mouse embryonic fibroblasts into adipocytes. It has been used in the histological analysis of rabbit′s eye and diabetic rat intestine.
Popular general purpose histological fixative.

Physical form

Neutral buffered solution

Certificate of Analysis

Certificate of Origin

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