TAQL-RO

Taq DNA Polymerase, 1 U/μl

suitable for PCR, hotstart: no, dNTPs included: no

• Synonym(s): dna amplification, pcr, polymerase, primer extension
• Enzyme Commission number: 2.7.7.7 (BRENDA, IUBMB)
• UNSPSC Code: 41106300

Properties

• biological source: bacterial (Thermus aquaticus BM)
• Quality Level: 100
• recombinant: expressed in E. coli
• form: liquid
• usage: sufficient for ≤2,000 reactions (11647687001); sufficient for ≤500 reactions (11647679001)
• specific activity: 1 U/μL
• mol wt: ~95 kDa
• feature: dNTPs included: no; hotstart: no
• packaging: pkg of 1,000 U (11647687001 [4 x 250 U]); pkg of 250 U (11647679001)
• manufacturer/tradename: Roche
• concentration: 25 U/mL; 30 U/mL
• parameter: 72 °C optimum reaction temp.
• technique(s): PCR: suitable
• color: colorless
• input: purified DNA
• optimum pH: ~9.0 (20 °C)
• solubility: water: miscible
• suitability: suitable for PCR; suitable for molecular biology
• NCBI accession no.: AAA27507; ; ; AAA27507
• UniProt accession no.: P19821
• application(s): genomic analysis; life science and biopharma
• foreign activity: Endonucleases 20 units, none detected; Nicking activity 20 units, none detected
• storage temp.: −20°C

Description

General description

The enzyme was cloned in E.coli and is isolated to be free of unspecific endo- or exonucleases according to the current quality control procedurese. Taq DNA Polymerase is a highly processive 5′–3′ DNA polymerase that lacks 3′–5′ exonuclease activity. It consists of a single polypeptide chain with a molecular weight of approximately 95 kDa. The enzyme exhibits highest activity at a pH of around 9 (adjusted at 20 °C) and temperatures around +75 °C. Taq DNA Polymerase also accepts modified deoxyribonucleosidetriphosphates as substrates, and can be used to label DNA-fragments either with radionucleotides, digoxigenin, fluorescein or biotin.
The high processivity, absence of exonuclease activity and temperature optima of Taq DNA Polymerase enable the use of this enzyme in DNA sequencing, especially where the resolution of secondary structures plays a major role.

Application

This lower concentration of our recombinant Taq DNA Polymerase allows small amounts of the polymerase to be pipetted more accurately and conveniently. In all other respects, this preparation is identical to our higher concentration (5 U/μl) preparation and can be used for:

• PCR
• RT-PCR
• Other primer-extension reactions, such as sequencing and labeling

Packaging

1 kit containing 4 components

Unit Definition

One unit Taq DNA Polymerase is defined as the amount of enzyme that incorporates 10 nmol of total deoxyribonucleoside triphosphates into acid precipitable DNA within 60 min at +65 °C under the assay conditions stated above.

Unit Assay: Incubation buffer:
67 mM Tris/HCl; pH 8.3/25 °C, 5 mM MgCl₂, 10 mM Mercaptoethanol, 0.2% Polydocanol, 0.2 mg/ml Gelatine, 0.2 mM each dATP, dGTP, dTTP and 0.1 mM dCTP.

Incubation procedure:
M13mp9ss, M13 primer (17mer) and 1 μCi (α^-32P) dCTP are incubated with suitable dilutions of Taq DNA Polymerase in 50 μl incubation buffer at +65 °C for 60 minutes. The amount of incorporated dNTPs is determined by trichloroacetic acid precipitation.

Volume Activity: 1 U/μl

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

Use of this product is covered by US patent claims and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patent claims require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

Kit Components Only

• Taq DNA Polymerase 1 U/μl
• PCR Buffer with MgCl₂ 10x concentrated
• MgCl₂ Stock Solution
• PCR Buffer without MgCl₂

Safety Information

• hcodes: H412
• pcodes: P273 - P501
• Hazard Classifications: Aquatic Chronic 3
• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 2
• flash_point_f: does not flash
• flash_point_c: does not flash