D1806

Sigma-Aldrich

Taq DNA Polymerase from Thermus aquaticus

with 10× PCR reaction buffer containing MgCl2

CAS Number:
Enzyme Commission number:
MDL number:
NACRES:
NA.55

Quality Level

recombinant

expressed in E. coli

form

liquid

usage

sufficient for 10000 reactions
sufficient for 3000 reactions
sufficient for 500 reactions

mol wt

94 kDa

feature

dNTPs included: no
hotstart: no

concentration

5 units/μL

application(s)

PCR: suitable

color

colorless

input

purified DNA

suitability

suitable for PCR and automated sequencing reactions

Featured Industry

Agriculture

shipped in

wet ice

storage temp.

−20°C

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Application

Taq DNA Polymerase from Thermus aquaticus has been used:
  • in the quantification of fungal growth by PCR (polymerase chain reaction) and photometric assay
  • in conventional RT(reverse transcriptase)-PCR
  • in SSR (simple sequence repeats) genotyping

Taq DNA Polymerase from Thermus aquaticus is a thermostable DNA polymerase that is used for the DNA polymerase chain reaction (PCR) in order to amplify DNA sequences.

Biochem/physiol Actions

Taq polymerase catalyzes oligonucleotide primer-driven, DNA template dependent incorporation of dNTPs into complimentary DNA strands. It displays both 5′ to 3′ polymerase and exonuclease activities.

Features and Benefits

  • Low per unit cost of Taq

Packaging

Taq DNA Polymerase with 10× reaction buffer containing MgCl2
Taq DNA polymerase comes with the choice of an optimized 10× reaction buffer including MgCl2 (D1806) or a 10× reaction buffer without MgCl2 plus a separate tube of MgCl2 for titration (D4545). The latter option may be necessary to determine optimal conditions for amplification.

Other Notes

Taq DNA Polymerase is a specialized thermostable enzyme isolated from the thermophilic bacterium Thermus aquaticus. The recombinant form of this enzyme is expressed in E. coli. This 94 kDa protein shows no detectable levels of contaminating endonucleases or exonucleases by SDS-PAGE. It has both 5′→3′ polymerase and exonuclease activity.

Unit Definition

One unit will incorporate 10 nmol of total dNTPs into acid-precipitable DNA in 30 min at 74 °C.

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.

storage_class_code

12 - Non Combustible Liquids

WGK Germany

WGK 1

Certificate of Analysis

Certificate of Origin

Unraveling the efficiency of RAPD and SSR markers in diversity analysis and population structure estimation in common bean
Zargar S M, et al.
Saudi Journal of Biological Sciences, 23(1), 139-149 (2016)
Fundamental study on the influence of Fusarium infection on quality and ultrastructure of barley malt
Oliveira P M, et al.
International Journal of Food Microbiology, 156(1), 32-43 (2012)
Lactic acid bacteria bioprotection applied to the malting process. Part II: Substrate impact and mycotoxin reduction
Oliveira P, et al.
Food Control, 51, 444-452 (2015)
Human endogenous retrovirus family HERV-K (HML-2) RNA transcripts are selectively packaged into retroviral particles produced by the human germ cell tumor line Tera-1 and originate mainly from a provirus on chromosome 22q11. 21
Ruprecht K, et al.
Journal of Virology, 82(20), 10008-10016 (2008)
Rita Horvath et al.
Brain : a journal of neurology, 129(Pt 7), 1674-1684 (2006-04-20)
Mutations in the gene coding for the catalytic subunit of the mitochondrial DNA (mtDNA) polymerase gamma (POLG1) have recently been described in patients with diverse clinical presentations, revealing a complex relationship between genotype and phenotype in patients and their families....
Articles
Learn about the history of the polymerase chain reaction (PCR), from the basic principles that proceeded its discovery to the awarding of a Nobel Prize for Chemistry and more recent developments such as real-time PCR (qPCR) and digital PCR.
Read More
Protocols
Hot Start dNTPs are modified with a thermolabile protecting group at the 3’ terminus. The presence of this modification blocks nucleotide incorporation by DNA polymerase until the nucleotide protecting group is removed during a heat activation step.
Read More
Learn standard PCR protocol steps and review reagent lists or cycling parameters. This method for routine PCR amplification of DNA uses standard Taq DNA polymerase.
Read More

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