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CORET

Sigma-Aldrich

PCR Core kit with Taq DNA polymerase

PCR components in separate tubes to allow for optimization

eCl@ss:
32161000
NACRES:
NA.55

Quality Level

form

liquid

usage

sufficient for 250 reactions

feature

dNTPs included
hotstart: no

technique(s)

PCR: suitable

color

colorless

input

purified DNA

shipped in

wet ice

storage temp.

−20°C

Related Categories

General description

Taq DNA Polymerase is a thermostable enzyme derived from the thermophilic bacterium Thermus aquaticus. This enzyme is in a recombinant form, expressed in E. coli. Taq DNA polymerase enzyme can withstand high temperatures up to 95 °C without significant loss of activity. Taq DNA Polymerase is a magnesium ion-dependent enzyme. Taq DNA Polymerase possesses 5′→3′ DNA polymerase activity and 5′→3′ exonuclease activity.

Application

CORET PCR kit has been used for the amplification of genomic DNA during the study of genetic diversity in various species of Manilkara zapota.[30]
PCR Core kit with Taq DNA polymerase is suitable for the amplification of genomic DNA, cDNA, or cloned DNA templates. Each component has been tested in the polymerase chain reaction. Optimal concentrations of Taq polymerase, template DNA, primers, and MgCl2 will depend on the specifics of the system. It may be necessary to optimize each component separately, especially the Taq and Mg2+ concentrations and the cycling parameters.

Quantity

Kit contains enough reagents for 250 units of Taq DNA polymerase.

Other Notes

The following reagents are required but not provided in the kit:
  • Mineral Oil, M 8662 (optional)
  • Thermocycler
  • Primers
  • DNA to be amplified
  • Chloroform, C 7559 (optional)

Legal Information

No license is conveyed with the purchase of this product under any of US Patents Nos. 5,804,375, 5,994,056, 6,171,785, 6,214,979, 5,538,848, 5,723,591, 5,876,930, 6,030,787, and 6,258,569, and corresponding patents outside the United States, or any other patents or patent applications, relating to the 5′ Nuclease and dsDNA-Binding Dye Processes. For further information contact the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

Kit Components Also Available Separately

Product No.
Description
SDS

  • P219210X PCR Buffer, Optimized for routine PCR with MgCl2 included 1 mL/vial

  • P231710X PCR Buffer without MgCl2, Optimized for routine PCR without MgCl2 1 mL/vial

  • M8787Magnesium chloride solution, PCR Reagent, 25 mM MgCI2 solution for PCR 1 mL/vial

  • W1754Water, PCR Reagent 3 vial(s)

  • D7295Deoxynucleotide Mix, 10 mM, Molecular Biology Reagent .25 mL

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Product Information Sheet

SDS

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