FPCRN-RO

Roche

FastStart Taq DNA Polymerase, dNTPack

Quality Level

usage

sufficient for ≤1,250 reactions (04738403001)
sufficient for ≤2,500 reactions (04738420001)
sufficient for ≤250 reactions (04738357001)
sufficient for ≤50 reactions (04738314001)
sufficient for ≤500 reactions (04738381001)

packaging

pkg of 1,000 U (04738381001 4x250 U)
pkg of 2,500 U (04738403001 10x250 U)
pkg of 5,000 U (04738420001 20x250 U)
pkg of 100 U (04738314001)
pkg of 500 U (04738357001 2x250 U)

mfr. no.

Roche

parameter

72 °C optimum reaction temp.

shipped in

dry ice

storage temp.

−20°C

General description

The enzyme dNTPack comprises FastStart Taq DNA Polymerase and a ready-to-use solution of PCR grade nucleotides. FastStart Taq DNA Polymerase is a versatile enzyme that can be used in a wide variety of applications and on multiple instrument platforms. This modified, thermostable, recombinant Taq DNA polymerase is inactive at temperatures below +75 °C, but is activated by a 2 to 4 minute heat activation step at +95 °C. Since it is inactive at low temperatures, FastStart Taq DNA Polymerase cannot elongate non-specific primer-template hybrids that may form at those temperatures. An optimized PCR buffer system and a GC-RICH Solution to enable the enzyme to handle a wide range of templates are also supplied.

Application

FastStart Taq DNA Polymerase is a thermostable, chemically modified form of recombinant Taq DNA Polymerase. The enzyme is inactive at +15 to +25°C during PCR setup, and then activated at +95°C during initial denaturation. This enzyme delivers superior results due to its unique enzyme design and optimized buffer system. FastStart Taq DNA Polymerase is an ideal tool for hot start PCR, because the enzyme remains inactive during PCR setup and prior to the initial denaturation step. It can be applied for PCR:
  • Hot start PCR up to 3 kb
  • Hot Start RT-PCR up to 3 kb
  • Multiplex PCR
  • Difficult templates, e.g., secondary structures or GC-rich sequences
  • Automated PCR, e.g., handling at room temperatures
  • quantitaive PCR(qPCR)

For maximum convenience, select the 2x concentrated ready-to-use FastStart PCR Master.

Features and Benefits

Volume activity: 5 U/μl
Each dNTPack contains 10 mM additive-free sodium salt nucleotides as a ready-to-use mix.

  • Higher specificity, sensitivity, and yield:
Hot start PCR makes PCR setup easier.
  • Use robotic setup.
Use this enyzme mix stable for 24 hours at +15 to +25°C.
  • Prevent PCR carryover contamination.
Incorporate dUTP and use Uracil-DNA Glycosylase to pretreat PCR master mixes.
  • Cost-effective.
Use the convenient premixed solution of PCR grade nucleotides.

Packaging

1 kit containing 6 components

Quality

Each lot is function-tested using human genomic DNA and primers specific for the 365 bp fragment of human tPA gene, and, a 284 bp fragment of the Apo E gene with 74% GC content. Each lot is also tested for the absence of exo- and endonucleases and nicking activity.
For details please refer to the respective Instruction for Use.

Unit Definition

One unit FastStart DNA Polymerase is defined as the amount of enzyme that incorporates 10 nmol of total deoxyribonucleosidetriphosphates into acid precipitable DNA within 30 minutes at +75 °C under the assay conditions stated under unit assay.

Unit Assay: 1 μg M13mp9ss DNA, 0.3 μg M13 sequencing primer and 0.1 μCi α--32P dCTP are incubated with varying amounts of units of FastStart Taq DNA Polymerase in 50 μl incubation buffer at 65 °C for 60 min. The amount of incorporated dNTPs is determined.

Volume Activity: 5 U/μl

Other Notes

For life science research only. Not for use in diagnostic procedures.

Hot start protocols improve PCR specificity, sensitivity, and yield. Heat-labile blocking groups make the modified enzyme inactive at +15 to +25°C. No elongation occurs when primers bind nonspecifically. The enzyme is activated by removing the blocking groups at +95°C for 2 to 6 minutes. PCR products with 3′-single A overhangs are produced, ideal for T/A cloning. For PCR carryover prevention, use the PCR Nucleotide MixPLUS and Uracil-DNA Glycosidase, heat-labile.

Legal Information

NOTICE TO PURCHASER: LIMITED LICENSE<br />Use of this product is covered by US Patent No. 6,127,155 and corresponding patent claims outside the US.  The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research.  No right under any other patent claim and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel.  This product is for research use only. Human and veterinary diagnostic uses under Roche patent claims require a separate license from Roche.  All uses other than internal research and human and veterinary diagnostic uses under Roche patent claims require a separate license from Thermo Fisher Scientific. Further information on purchasing licenses from Roche may be obtained by contacting the Licensing Department of Roche Molecular Systems, Inc., 4300 Hacienda Drive, Pleasanton, California 94588, USA or Roche Diagnostics GmbH, Sandhofer Strasse 116, 68305 Mannheim, Germany.  Further information on purchasing licenses from Thermo Fisher Scientific may be obtained by contacting the Licensing Department of  Thermo Fisher Scientific, 5791 Van Allen Way, Carlsbad, California 92008, USA.</ br>NOTICE TO PURCHASER: LIMITED LICENSE<br />Use of this product is covered by US Patent Nos. 5,677,152 and 5,773,258, and corresponding patent claims outside the US.  The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research.  No right under any other patent claim and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel.  This product is for research use only.  Human and veterinary diagnostic uses under Roche patent claims require a separate license from Roche.  All uses other than internal research and human and veterinary diagnostic uses under Roche patent claims require a separate license from Thermo Fisher Scientific. Further information on purchasing licenses from Roche may be obtained by contacting the Licensing Department of Roche Molecular Systems, Inc., 4300 Hacienda Drive, Pleasanton, California 94588, USA or Roche Diagnostics GmbH, Sandhofer Strasse 116, 68305 Mannheim, Germany.  Further information on purchasing licenses from Thermo Fisher Scientific may be obtained by contacting the Licensing Department of  Thermo Fisher Scientific, 5791 Van Allen Way, Carlsbad, California 92008, USA.
FastStart is a trademark of Roche

Kit Components Only

Product No.
Description

  • FastStart Taq DNA Polymerase, in storage and dilution buffer 5 U/μl

  • PCR Reaction Buffer, with 20 mM MgCl2 10x concentrated

  • PCR Reaction Buffer, without MgCl2 10x concentrated

  • MgCl2 Stock Solution 25 mM

  • GC-RICH Solution 5x concentrated

  • PCR Nucleotide Mix

RIDADR

NONH for all modes of transport

Certificate of Analysis

Certificate of Origin

Melanie A Jones et al.
Genetics in medicine : official journal of the American College of Medical Genetics, 13(11), 921-932 (2011-08-04)
Congenital disorders of glycosylation are a heterogeneous group of disorders caused by deficient glycosylation, primarily affecting the N-linked pathway. It is estimated that more than 40% of congenital disorders of glycosylation patients lack a confirmatory molecular diagnosis. The purpose of...
Analysis of maternal microchimerism in rhesus monkeys (Macaca mulatta) using real-time quantitative PCR amplification of MHC polymorphisms
Miura M et al.
Wellcome open research, 3 (2018)
Analysis of maternal microchimerism in rhesus monkeys (Macaca mulatta) using real-time quantitative PCR amplification of MHC polymorphisms
Bakkour S, et al.
Chimerism, 5, 615-615 (2014)
Spontaneous HTLV-1 transcription is accompanied by distinct epigenetic changes in the 5' and 3' long terminal repeats
Wellcome open research, 3 (2018)
Sonia Bakkour et al.
Chimerism, 5(1), 6-15 (2014-01-24)
Although pregnancy-associated microchimerism is known to exist in humans, its clinical significance remains unclear. Fetal microchimerism has been documented in rhesus monkeys, but the trafficking and persistence of maternal cells in the monkey fetus and infant have not been fully...
Articles
The choice of the PCR enzyme in combination with an appropriate buffer can profoundly affect PCR outcome.
Read More
The purpose of Hot Start PCR is to inhibit the PCR reaction in order to reduce nonspecific amplification, prevent the formation of primer dimers, and increase product yields.
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Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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