8.05740

2-Mercaptoethanol

for synthesis

• Synonym(s): Thioethylene glycol, Thioglycol, β-Mercaptoethanol, 2-Hydroxyethylmercaptan, BME, Thioethylene glycol
• Linear Formula: HSCH₂CH₂OH
• CAS Number: 60-24-2
• Molecular Weight: 78.13
• Beilstein: 773648
• MDL number: MFCD00004890
• EC Index Number: 200-464-6

Properties

• vapor density: 2.69 (vs air)
• Quality Level: 200
• vapor pressure: 1 mmHg ( 20 °C)
• assay: ≥99% (GC)
• form: liquid
• autoignition temp.: 295 °C
• potency: 98-162 mg/kg LD₅₀, oral (Rat); 112-224 mg/kg LD₅₀, skin (Rabbit)
• expl. lim.: 18 %
• reaction suitability: reagent type: reductant
• concentration: 14.3 M (pure liquid)
• refractive index: n20/D 1.500 (lit.)
• pH: 4.5-6 (20 °C, 500 g/L in H₂O)
• bp: 157 °C (lit.)
• mp: <-50 °C
• transition temp: flash point 70.5 °C
• density: 1.114 g/mL at 25 °C (lit.)
• storage temp.: 2-30°C
• SMILES string: OCCS
• InChI: 1S/C2H6OS/c3-1-2-4/h3-4H,1-2H2
• InChI key: DGVVWUTYPXICAM-UHFFFAOYSA-N

Description

Application

BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.

Safety Information

• Pictograms: GHS05,GHS06,GHS08,GHS09
• Signal Word: Danger
• Hazard Statements: H301 + H331 - H310 - H315 - H317 - H318 - H361d - H373 - H410
• Precautionary Statements: P273 - P280 - P301 + P310 - P302 + P352 + P310 - P304 + P340 + P311 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1A - STOT RE 2 Oral
• Storage Class Code: 6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials
• WGK: WGK 3
• Flash Point(F): 165.2 °F - closed cup
• Flash Point(C): 74 °C - closed cup