444203

β-Mercaptoethanol, Molecular Biology Grade

A disulfide-reducing agent.

• Synonym(s): 2-Mercaptoethanol, BME, β-Mercaptoethanol, 2-Hydroxyethylmercaptan, Thioethylene glycol
• Linear Formula: HSCH₂CH₂OH
• CAS Number: 60-24-2
• Molecular Weight: 78.13
• Beilstein/REAXYS Number: 773648
• MDL number: MFCD00004890
• UNSPSC Code: 12161504
• NACRES: NA.25

Properties

• grade: Molecular Biology
• Quality Level: 100
• vapor density: 2.69 (vs air)
• vapor pressure: 1 mmHg ( 20 °C)
• assay: ≥99% (GC)
• form: liquid
• expl. lim.: 18 %
• reaction suitability: reagent type: reductant
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• concentration: 14.3 M (pure liquid)
• refractive index: n20/D 1.500 (lit.)
• pH: 4.5-6 (20 °C, 500 g/L)
• bp: 157 °C (lit.)
• mp: <-50 °C
• density: 1.12 g/cm³ at 20 °C; 1.114 g/mL at 25 °C (lit.)
• cation traces: heavy metals (as Pb): ≤0.0001%
• absorption: ≤0.31 at 280 nm; ≤1.50 at 260 nm
• foreign activity: DNases, proteases, RNases, none detected
• shipped in: ambient
• storage temp.: 15-25°C
• SMILES string: OCCS
• InChI: 1S/C2H6OS/c3-1-2-4/h3-4H,1-2H2
• InChI key: DGVVWUTYPXICAM-UHFFFAOYSA-N

Description

General description

A disulfide-reducing agent.

A disulfide-reducing agent. Reagent commonly used in organic synthesis (aborsorbance: ≤1.50 at 260 nm; ≤0.31 at 280 nm).

Application

BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.

Warning

Toxicity: Toxic (F)

Other Notes

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• pictograms: GHS06,GHS08,GHS05,GHS09
• signalword: Danger
• hcodes: H301 + H331,H310,H315,H317,H318,H361f,H373,H410
• pcodes: P273 - P280 - P301 + P310 - P302 + P352 + P310 - P304 + P340 + P311 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1A - STOT RE 2 Oral
• target_organs: Liver,Heart
• Storage Class: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• wgk_germany: WGK 3
• flash_point_f: 165.2 °F - closed cup
• flash_point_c: 74 °C - closed cup