MilliporeSigma
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137151

SAFC

2-Mercaptoethanol

EMPROVE® EVOLVE

Synonym(s):
β-Mercaptoethanol, 2-Hydroxyethylmercaptan, BME, Thioethylene glycol
Linear Formula:
HSCH2CH2OH
CAS Number:
Molecular Weight:
78.13
Beilstein:
773648
MDL number:

vapor density

2.69 (vs air)

Quality Level

vapor pressure

1 mmHg ( 20 °C)

assay

99.0-101.0% (w/w)

form

liquid

expl. lim.

18 %

concentration

14.3 M (pure liquid)

impurities

≤1.0% related substances (GC) 2,2-Dithiodiethanol

refractive index

n20/D 1.500 (lit.)

bp

157 °C (lit.)

density

1.114 g/mL at 25 °C (lit.)

suitability

corresponds for identity (IR)

storage temp.

2-8°C

SMILES string

OCCS

InChI

1S/C2H6OS/c3-1-2-4/h3-4H,1-2H2

InChI key

DGVVWUTYPXICAM-UHFFFAOYSA-N

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Application

BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.

Analysis Note

purity (GC) ≥99.0%

Legal Information

Emprove is a registered trademark of Merck KGaA, Darmstadt, Germany

Signal Word

Danger

Hazard Classifications

Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2 Oral

Storage Class Code

6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

Flash Point(F)

165.2 °F - closed cup

Flash Point(C)

74 °C - closed cup

Certificate of Analysis

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Certificate of Quality

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