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480275

Sigma-Aldrich

β-NGF, Human, Recombinant, NSO Cells

Synonym(s):

β-Nerve Growth Factor, Human, Recombinant

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.75

recombinant

expressed in NSO cells

Quality Level

assay

≥97% (SDS-PAGE)

form

lyophilized

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

impurities

≤100 pg/μg Endotoxin (pg/μg β-NGF)

shipped in

ambient

storage temp.

−70°C

General description

Recombinant, human β-nerve growth factor expressed in NSO cells. Involved in neuroimmune interactions and inflammation.

Biochem/physiol Actions

ED₅₀ = 800-1500 pg/ml as measured in a cell proliferation assay using a factor-dependent human erythroleukemic cell line

Warning

Toxicity: Standard Handling (A)

Physical form

Lyophilized from sterile-filtered solution in PBS containing 50 µg BSA/µg β-NGF.

Reconstitution

Following reconstitution, aliquot and freeze (-70°C). Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 3 months at -70°C.
Reconstitute to a concentration ≥1 µg/ml with sterile PBS containing at least 0.1% HSA or BSA.

Other Notes

Leon, A., et al. 1994. Proc. Natl. Acad. Sci. USA91, 3739.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3


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