N2513
NGF-β from rat
recombinant, expressed in Sf21 cells, lyophilized powder, suitable for cell culture
Synonym(s):
Nerve Growth Factor-β from rat
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About This Item
Recommended Products
biological source
rat
Quality Level
recombinant
expressed in Sf21 cells
assay
≥97% (SDS-PAGE)
form
lyophilized powder
potency
0.15-3.00 ng/mL EC50
packaging
pkg of 0.1 mg
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
solubility
water: soluble 0.1 g/L at 20 °C
UniProt accession no.
storage temp.
−20°C
Gene Information
rat ... NGF(4803)
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General description
NGFβ (nerve growth factor β) is a protein involved in signal transduction, and expressed by multiple tissues such as placenta, urothelium and bladder smooth muscle cells. It is a neurotrophin and belongs to the NGF family.
Application
NGF-β from rat is used for the following applications:
- In the preparation of DMEM medium (for the cultivation and differentiation of PC12 cells)
- Western blotting analysis
- For the preparation of differentiation medium (it is prepared from DMEM, in which NGF-β is one of the components)
- In cell culture and treatment (for neuronal differentiation)
- In the preparation of stock solution
Biochem/physiol Actions
NGFβ (nerve growth factor β) is essential for the differentiation and survival of neurons. It also plays a key role in the development and maintenance of nervous system. It is also involved in angiogenesis, and facilitates tumorigenesis such as, oral, breast and prostate cancers. It has potential as marker for the diagnosis of interstitial cystitis/painful bladder syndrome (IC/PBS) and overactive bladder (OAB), and might also aid in determining the therapy for the same. It might be involved in changes in placental function, as NGF signaling is influenced by antenatal depression and selective serotonin reuptake inhibitor (SSRI) treatment.
Physical form
Lyophilized from a 0.2 μm filtered solution in PBS and 1 M NaCl containing 50 μg of bovine serum albumin per 1 μg of cytokine
Analysis Note
The biological activity is measured in a cell proliferation assay using the factor-dependent human erythroleukemic cell line, TF-1.
Storage Class
13 - Non Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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