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一般說明
應用
- 用于需要减少非特异性扩增的 PCR 扩增
- 用于多重 PCR
- 用于减少引物二聚体
特點和優勢
- 该预混液可确保反应间一致性
- 缩短了准备时间，并减少了多个移液步骤造成的污染风险
- 可最大限度减少非特异性扩增和污染
- 提高了特异性和靶标产量
- 减少了引物二聚体
- 与手动或蜡质热启动方法相比，准备时间更短
- 允许室温反应
- 非常适合高通量定量PCR应用
包裝
100个反应包装为1×2.5mL
400个反应包装为1×10mL
其他說明
本品已通过定量PCR验证，但可能需要补充25 mM氯化镁溶液（货号M8787）、适宜的荧光探针以及（如果需要）内参染料（货号R4526）。请在www.sigma-aldrich.com/hotstart页面查看有关JumpStart Taq ReadyMix产品的更多详细信息。
法律資訊
相關產品
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
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文章
Method outlines use of a hot start Taq for multiplex qPCR and provides guidance on how to optimize dNTPs, primer, probes and MgCL2 concentrations. By optimizing these parameters, the user can improve assay sensitivity and linear range of detection.
Learn about the history of the polymerase chain reaction (PCR), from the basic principles that proceeded its discovery to the awarding of a Nobel Prize for Chemistry and more recent developments such as real-time PCR (qPCR) and digital PCR.
Real-time polymerase chain reaction allows researchers to estimate the quantity of starting material in a sample. It has a much wider dynamic range of analysis than conventional PCR
Real-time polymerase chain reaction allows researchers to estimate the quantity of starting material in a sample. It has a much wider dynamic range of analysis than conventional PCR
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The CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) system was discovered in bacteria, where it functions as an adaptive immune system against invading viral and plasmid DNA.
Protocol using antibody mediated hot start polymerase. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.
When using hot start Taq DNA polymerase, the enzyme remains inactive until heated. Hot Start DNA polymerase control is achieved by chemical or antibody modification of the enzyme.
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