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重要文件

T9424

Sigma-Aldrich

TRI试剂®

For processing tissues, cells cultured in monolayer or cell pellets

同義詞:

TRI试剂® RNA 分离试剂

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About This Item

MDL號碼:
分類程式碼代碼:
12352200
NACRES:
NA.52

暫時無法取得訂價和供貨情況

品質等級

用途

 mL sufficient for 107 cells
 mL sufficient for 100 mg tissue (or)

儲存溫度

2-8°C

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一般說明

TRI reagent®是一种快速方便的即用型试剂,可实现有效的总RNA提取或同时分离RNA、DNA和蛋白质。TRI reagent®是溶于单相溶液的硫氰酸胍和苯酚混合物。
组织样品在TRI reagent®中均质或裂解,即可溶解RNA、DNA和蛋白质。加入氯仿或1-溴-3-氯丙烷,然后离心,混合物将分离为三层:含RNA的水相、含DNA的中间相和含蛋白质的有机相。随后即可通过分离不同相层,得到相应组分。

應用

TRI Reagent已用于:
  • 从各种组织样品和细胞中分离总RNA [1][2][3][4]
  • 裂解单核细胞 [5]
  • 从感染SARS-CoV的Vero细胞中分离细胞质RNA [6]
  • 从感染甲型流感(H5N1)病毒的MDM(单核细胞衍生的巨噬细胞)中分离总RNA [7]
  • 制备SARS-CoV培养物的病毒裂解液,以便为SARS-CoV疫苗开发创建编码S糖蛋白的克隆 [8]

使用TRI reagent®分离的RNA、DNA和蛋白质可继续用于各种下游应用,如克隆、PCR、RT-PCR、Northern印迹、mRNA分离、体外翻译、RNase保护测定、限制酶消化、Southern印迹、SDS-PAGE和蛋白质印迹。
TRI试剂是对由Chomczynski开发的一步法总RNA分离试剂进行改良的一种版本。基于该试剂的RNA分离方法已被广泛用于和验证用于RNA应用。它是对来自人、动物、植物、酵母、细菌和病毒来源样本进行总RNA或RNA、DNA和蛋白同时分离的一种理想的快速、经济和高效的方法。

特點和優勢

易于使用:通过一种试剂可同时分离RNA、DNA和蛋白质,不会残留任何污染物。
多用途:TRI Reagent®同时适于大量或少量组织或细胞,同时支持多种类型的样品,包括人、植物、酵母、细菌和病毒来源样品。
高效:相对于传统硫氰酸胍/氯化铯方法,TRI reagent®产量更高。从新鲜组织或细胞开始的完整RNA提取流程可在一小时内完成。
  • 可轻松扩展的RNA分离
  • 可适用于多种来源:人、植物、酵母、细菌或病毒
  • 相对于传统的硫氰酸胍/氯化铯具有更高的产量

法律資訊

TRI Reagent is a registered trademark of Molecular Research Center, Inc.

相關產品

產品號碼
描述
訂價

訊號詞

Danger

危險分類

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2

標靶器官

Nervous system,Kidney,Liver,Skin

安全危害

儲存類別代碼

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

水污染物質分類(WGK)

WGK 2

閃點(°F)

174.2 °F - closed cup

閃點(°C)

79 °C - closed cup


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Cultrex™ 3-D Culture Cell Harvesting Kit provides an optimized and standardized solution for the isolation and normalization of cell lysates from 3-D Culture Matrix™ BME or Laminin I for subsequent biochemical analysis.

The availability of simple methods for purification of DNA and RNA has greatly facilitated the analysis and characterization of the genome and gene expression. There is a demand to isolate DNA and RNA rapidly and conveniently from a variety of cellular sources, including cells and tissues from mammalian, plant and bacterial cultures.

條款

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相關內容

An overview of cell lysis and protein extraction methods including detergent solubilization, freeze-thaw lysis, osmotic shock, sonication, enzymatic cell lysis, and mechanical disruption techniques such as Dounce, Polytron, and mortar and pestle homogenization.

An overview of cell lysis and protein extraction methods including detergent solubilization, freeze-thaw lysis, osmotic shock, sonication, enzymatic cell lysis, and mechanical disruption techniques such as Dounce, Polytron, and mortar and pestle homogenization.

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