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Merck
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文件

P0982

Sigma-Aldrich

JumpStart™ REDTaq® ReadyMix™ 反应混合物

for PCR

同義詞:

Hot start PCR master mix, Hot start master mix, Hot start taq master mix

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About This Item

MDL號碼:
MFCD00166026
分類程式碼代碼:
41106300

品質等級

200

形狀

liquid

用途

sufficient for 100 reactions
 sufficient for 20 reactions
 sufficient for 800 reactions

特點

dNTPs included
hotstart

濃度

2.5 units/reaction (50 μL reaction volume)

技術

PCR: suitable

顏色

red

輸入

purified DNA

應用

agriculture

運輸包裝

wet ice

儲存溫度

−20°C

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相關類別

一般說明

JumpStart REDTaq ReadyMix PCR反应混合物是制备好的溶液，结合了热启动PCR的性能优势和Sigma ReadyMix和REDTaq的双重便利性。反应混合物优化的2x反应浓缩物中包括Sigma的JumpStart Taq DNA聚合酶、99％纯度的脱氧核苷酸、缓冲液和惰性红色染料。将25 μL ReadyMix加到引物、模板和水中，使最终反应体积为50 μL。在室温下，反应混合物中的JumpStart Taq抗体可使Taq DNA聚合酶失活。在PCR的第一次变性步骤期间，复合物解离，聚合酶活性完全恢复。惰性红色染料不影响自动测序、限制性酶切、连接或其他下游操作。如果需要，只需通过标准纯化方法就可将PCR产物与染料轻松分离。热启动机制允许设置室温，使其成为高通量应用的理想选择 。

應用

Jumpstart™ REDTaq® ReadyMix™反应混合物可用于直接提取DNA并进行PCR扩增。还用于：
  • 多重定量PCR
  • 甲基化特异性PCR(MSPCR)
  • 总RNA合成cDNA的PCR扩增

特點和優勢

  • JumpStart™ REDTaq® DNA聚合酶是一种抗体失活的热启动酶，旨在最大限度地减少非特异性扩增，同时提高目标产量和特异性
  • 无论模板浓度如何，JumpStart Taq DNA聚合酶均可提供优越的扩增效果
  • REDTaq JumpStart ReadyMix可减少移液步骤，降低污染风险。 热启动机制允许设置室温，使其成为高通量应用的理想选择 。
  • 惰性红色染料方便验证试剂是否适当混合
  • 不需要装载其他染料。可以在反应后将等分试样直接加载到琼脂糖凝胶上

包裝

默认的反应体积为50 μL

20RXN包装成1 X 500 μL
100RXN包装成1 X 2.5 mL
800RXN包装成1 X 20 mL

單位定義

在74℃下，一个单位可在30分钟内将10 nM总dNTP掺入酸可沉淀的DNA中。

其他說明

想查看JumpStart REDTaq和ReadyMix产品的更多详细信息，请访问 www.sigma-aldrich.com/hotstart。
对于典型的PCR反应，将25 μL JumpStart™ REDTaq® ReadyMix™反应混合物与25 μL混合物（含有模板DNA、引物和水）混合。可根据需要改变反应体积。

法律資訊

凡购买本产品，即表示授权用户使用产品说明书所指定的专利，但仅限于将所购数量的产品用于内部研究用途。我们未明确表示、暗示，或通过禁止反言形式转让任何其他专利权（如，5′ Nuclease Process专利权）。有关购买许可的更多信息，可联系Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA获取。
依照美国专利号5,338,671和5,587,287和其他地区对应的专利，许可抗体用于体外研究用途。
本产品的使用受到如下一项或多项美国专利及其对应的境外专利声明保护：US 8,404,464和US 7,972,828。 购买本产品，即相当于依照境外的专利声明获得了一份受限制、不可转让的使用许可。
JumpStart is a trademark of Sigma-Aldrich Co. LLC
REDTaq is a registered trademark of Merck KGaA, Darmstadt, Germany
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。

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Nijiro Nohata et al.
Developmental biology, 411(2), 183-194 (2016-02-14)
Angiogenesis, the formation of new blood vessels by remodeling and growth of pre-existing vessels, is a highly orchestrated process that requires a tight balance between pro-angiogenic and anti-angiogenic factors and the integration of their corresponding signaling networks. The family of
Stephen J Murphy et al.
DNA research : an international journal for rapid publication of reports on genes and genomes, 19(5), 395-406 (2012-09-20)
High-throughput next-generation sequencing provides a revolutionary platform to unravel the precise DNA aberrations concealed within subgroups of tumour cells. However, in many instances, the limited number of cells makes the application of this technology in tumour heterogeneity studies a challenge.
Stephen J Murphy et al.
Gastroenterology, 145(5), 1098-1109 (2013-08-06)
Increasing grade of pancreatic intraepithelial neoplasia (PanIN) has been associated with progression to pancreatic ductal adenocarcinoma (PDAC). However, the mechanisms that control progression from PanINs to PDAC are not well understood. We investigated the genetic alterations involved in this process. Genomic
Avery Sicher et al.
Neurotoxicology and teratology, 85, 106962-106962 (2021-02-27)
Public health campaigns broadcast the link between heavy alcohol consumption during pregnancy and physical, cognitive, and behavioral birth defects; however, they appear less effective in deterring moderate consumption prevalent in women who are pregnant or of childbearing age. The incidence
The role of formyl peptide receptor 1 (FPR1) in neuroblastoma tumorigenesis
Snapkov I, et al.
BMC Cancer, 16, 490-490 (2016)
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條款

Applications with REDTaq™

Reviews the applications and benefits for RedTaq, including standard RedTaq, Hot Start RedTaq and RedTaq for genomic DNA PCR.

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